Functional analysis of Arl1 and golgin-97 in endosome-to-TGN transport using recombinant Shiga toxin B fragment.

A direct transport route from early/recycling endosome (EE/RE) to the trans-Golgi network (TGN) is exploited by Shiga toxin (Mallard et al., 1998) and TGN38 (Ghosh et al., 1998). To facilitate the study of this pathway, both in vivo and in vitro transport assays using recombinant Shiga toxin B fragments (STxB) as protein cargos have facilitated the analysis of this transport event (Johannes et al., 1997; Mallard et al., 1998, 2002; Tai et al., 2004). We describe here the application of these assays to study the role of a small GTPase Arl1 and its effector golgin-97 in this transport process.