Literature DB >> 16413261

Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size.

Joëlle Bigay1, Bruno Antonny.   

Abstract

The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16413261     DOI： 10.1016/S0076-6879(05)04010-3

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  16 in total

1.  Membrane curvature induced by Arf1-GTP is essential for vesicle formation.

Authors:  Rainer Beck; Zhe Sun; Frank Adolf; Chistoph Rutz; Jochen Bassler; Klemens Wild; Irmgard Sinning; Ed Hurt; Britta Brügger; Julien Béthune; Felix Wieland
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-08       Impact factor: 11.205

2.  Synaptojanin 1-mediated PI(4,5)P2 hydrolysis is modulated by membrane curvature and facilitates membrane fission.

Authors:  Belle Chang-Ileto; Samuel G Frere; Robin B Chan; Sergey V Voronov; Aurélien Roux; Gilbert Di Paolo
Journal:  Dev Cell       Date:  2011-02-15       Impact factor: 12.270

3.  Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication.

Authors:  Thomas Kupke; Leontina Di Cecco; Hans-Michael Müller; Annett Neuner; Frank Adolf; Felix Wieland; Walter Nickel; Elmar Schiebel
Journal:  EMBO J       Date:  2011-07-22       Impact factor: 11.598

4.  9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments.

Authors:  Svetlana O Dodonova; Patrick Aderhold; Juergen Kopp; Iva Ganeva; Simone Röhling; Wim J H Hagen; Irmgard Sinning; Felix Wieland; John A G Briggs
Journal:  Elife       Date:  2017-06-16       Impact factor: 8.140

5.  FisB mediates membrane fission during sporulation in Bacillus subtilis.

Authors:  Thierry Doan; Jeff Coleman; Kathleen A Marquis; Alex J Meeske; Briana M Burton; Erdem Karatekin; David Z Rudner
Journal:  Genes Dev       Date:  2013-02-01       Impact factor: 11.361

6.  High yield production of myristoylated Arf6 small GTPase by recombinant N-myristoyl transferase.

Authors:  Dominique Padovani; Mahel Zeghouf; José A Traverso; Carmela Giglione; Jacqueline Cherfils
Journal:  Small GTPases       Date:  2013-01-01

7.  Liposome Flotation Assays for Phosphoinositide-protein Interaction.

Authors:  Helene Tronchere; Frederic Boal
Journal:  Bio Protoc       Date:  2017-03-05

8.  Discrete determinants in ArfGAP2/3 conferring Golgi localization and regulation by the COPI coat.

Authors:  Lena Kliouchnikov; Joëlle Bigay; Bruno Mesmin; Anna Parnis; Moran Rawet; Noga Goldfeder; Bruno Antonny; Dan Cassel
Journal:  Mol Biol Cell       Date:  2008-12-24       Impact factor: 4.138

9.  Insights into the mechanisms of membrane curvature and vesicle scission by the small GTPase Sar1 in the early secretory pathway.

Authors:  Hanaa Hariri; Nilakshee Bhattacharya; Kerri Johnson; Alex J Noble; Scott M Stagg
Journal:  J Mol Biol       Date:  2014-09-02       Impact factor: 5.469

10.  Crystal structure of a lipoxygenase from Cyanothece sp. may reveal novel features for substrate acquisition.

Authors:  Julia Newie; Alexandra Andreou; Piotr Neumann; Oliver Einsle; Ivo Feussner; Ralf Ficner
Journal:  J Lipid Res       Date:  2015-12-14       Impact factor: 5.922
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.