Effect of culture conditions on fatty acids composition of a biosurfactant produced by Candida ingens and changes of surface tension of culture media.

This work investigated the effect of culture medium composition on a biosurfactant production and their total fatty acids content, as well as the surface tension of media, and biomass production by Candida ingens. A factorial experimental design was used to evaluate the combined effect of C/P, C/N(inorganic), C/Fe, C/Mg ratios and yeast extract concentration. The highest biosurfactant production was reached when high C/Fe and high C/P ratio variables were combined; biosurfactant concentration increased by a 3.42 fold. The variable with the highest effect on net decrease surface tension (DeltaST) and fatty acids percentage of C. ingens biosurfactant was yeast extract. The average of DeltaST (25 mN/m) and fatty acids percentage (34.7%) values were enhanced at high yeast extract concentration of 1g/l. The main conclusion of this study was that the culture composition affected the biosurfactant production by C. ingens. It was also observed that the surface tension and total fatty acids of the biosurfactant were modified as the media composition changed.