Literature DB >> 16412439

Stoichiometry of lipid-protein interaction assessed by hydrophobic photolabeling.

Ana María Villamil Giraldo1, Pablo Raúl Castello, F Luis González Flecha, Jesper V Moeller, José María Delfino, Juan Pablo F C Rossi.   

Abstract

Here we undertook a comparative study of the composition of the lipid annulus of three ATPases pertaining to the P-type family: plasma membrane calcium pump (PMCA), sarcoplasmic reticulum calcium pump (SERCA) and Na,K-ATPase. The photoactivatable phosphatidylcholine analogue [(125)I]TID-PC/16 was incorporated into mixtures of dimyristoyl phosphatidylcholine (DMPC) and each enzyme with the aid of the nonionic detergent C(12)E(10). After photolysis, the extent of the labeling reaction was assessed to determine the lipid:protein stoichiometry: 17 for PMCA, 18 for SERCA, 24 for the Na,K-ATPase (alpha-subunit) and 5.6 mol PC/mol protein for the Na,K-ATPase (beta-subunit).

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Year:  2006        PMID： 16412439     DOI： 10.1016/j.febslet.2005.12.078

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  Mitochondrial Redox Opto-Lipidomics Reveals Mono-Oxygenated Cardiolipins as Pro-Apoptotic Death Signals.

Authors:  Gaowei Mao; Feng Qu; Claudette M St Croix; Yulia Y Tyurina; Joan Planas-Iglesias; Jianfei Jiang; Zhentai Huang; Andrew A Amoscato; Vladimir A Tyurin; Alexandr A Kapralov; Amin Cheikhi; John Maguire; Judith Klein-Seetharaman; Hülya Bayır; Valerian E Kagan
Journal:  ACS Chem Biol       Date:  2016-01-05       Impact factor: 5.100

2.  Conformational changes produced by ATP binding to the plasma membrane calcium pump.

Authors:  Irene C Mangialavori; Mariela S Ferreira-Gomes; Nicolás A Saffioti; Rodolfo M González-Lebrero; Rolando C Rossi; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2013-09-11       Impact factor: 5.157

3.  Autoinhibition mechanism of the plasma membrane calcium pump isoforms 2 and 4 studied through lipid-protein interaction.

Authors:  Irene C Mangialavori; Gerardo Corradi; Débora E Rinaldi; María Candelaria de la Fuente; Hugo P Adamo; Juan Pablo F C Rossi
Journal:  Biochem J       Date:  2012-04-01       Impact factor: 3.857

4.  Plasma membrane calcium pump (PMCA) differential exposure of hydrophobic domains after calmodulin and phosphatidic acid activation.

Authors:  Irene Mangialavori; Ana María Villamil-Giraldo; María F Pignataro; Mariela Ferreira-Gomes; Ariel J Caride; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2011-03-31       Impact factor: 5.157

5.  Protein-phospholipid interplay revealed with crystals of a calcium pump.

Authors:  Yoshiyuki Norimatsu; Kazuya Hasegawa; Nobutaka Shimizu; Chikashi Toyoshima
Journal:  Nature       Date:  2017-05-03       Impact factor: 49.962

6.  Diving Into the Lipid Bilayer to Investigate the Transmembrane Organization and Conformational State Transitions of P-type Ion ATPases.

Authors:  Irene C Mangialavori; Ariel J Caride; Rolando C Rossi; Juan Pablo F C Rossi; Emanuel E Strehler
Journal:  Curr Chem Biol       Date:  2011-05

7.  A new conformation in sarcoplasmic reticulum calcium pump and plasma membrane Ca2+ pumps revealed by a photoactivatable phospholipidic probe.

Authors:  Irene Mangialavori; Ana María Villamil Giraldo; Cristina Marino Buslje; Mariela Ferreira Gomes; Ariel J Caride; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2008-12-12       Impact factor: 5.157

8.  Determination of the dissociation constants for Ca2+ and calmodulin from the plasma membrane Ca2+ pump by a lipid probe that senses membrane domain changes.

Authors:  Irene Mangialavori; Mariela Ferreira-Gomes; María F Pignataro; Emanuel E Strehler; Juan Pablo F C Rossi
Journal:  J Biol Chem       Date:  2009-11-05       Impact factor: 5.157
  8 in total

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