Literature DB >> 16406130

Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.

C R Marutha Ravindran1, S Suguna, S Shanmugasundaram.   

Abstract

To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.

Mesh:

Year:  2006        PMID: 16406130     DOI: 10.1016/j.mimet.2005.11.011

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  3 in total

1.  Transformation and conjugal transfer of foreign genes into the filamentous multicellular cyanobacteria (subsection V) Fischerella and Chlorogloeopsis.

Authors:  Karina Stucken; Judith Ilhan; Mayo Roettger; Tal Dagan; William F Martin
Journal:  Curr Microbiol       Date:  2012-07-26       Impact factor: 2.188

2.  Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria.

Authors:  Arnaud Taton; Federico Unglaub; Nicole E Wright; Wei Yue Zeng; Javier Paz-Yepes; Bianca Brahamsha; Brian Palenik; Todd C Peterson; Farzad Haerizadeh; Susan S Golden; James W Golden
Journal:  Nucleic Acids Res       Date:  2014-07-29       Impact factor: 16.971

3.  Natural transformation of the filamentous cyanobacterium Phormidium lacuna.

Authors:  Fabian Nies; Marion Mielke; Janko Pochert; Tilman Lamparter
Journal:  PLoS One       Date:  2020-06-12       Impact factor: 3.240

  3 in total

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