Identification of family relationships by epigenetic traits.

In analyzing family burials, it is often necessary to establish the nature of the family relationship. This study examines 18 skulls from the 14th and 15th century, presumptively assigned to the family of the Counts of Celje from the territory of present-day Slovenia. Though DNA analysis is the identification method of choice, it is not always possible to apply it. In our case we were unable to isolate the genetic material and had to use classical identification methods. After determining sex and age, we established the kinship by comparison of X-ray images of paranasal cavities (frontal and maxillary sinuses, and also orbital and nasal cavities), the shape and size of which are autosomal dominant inherited characters. The comparison also extends to numerous other likewise inherited epigenetic trait similarities on the skulls. We identified all skulls, compared them with historical and genealogical data, grouped them into nuclear families--one of them is presented in this article-, and proved that they belong to the assigned family. This multidisciplinary work, based on latest epigenetic research, is highly relevant for modern non-genetic identification studies and verification of kinship by skulls collectively interred and can be applied in similar cases where isolation of DNA is no longer possible, even though the skeletal remains may not be old.