PURPOSE: To measure glucose flux across human, bovine, and porcine corneas and to determine the diffusion coefficient of each type of cornea. METHODS: Diffusion of glucose across human (n = 8), bovine (n = 7), and pig corneas (n = 8) was measured using a modified blind well chamber apparatus (Boyden chamber). Dialysis membranes (n = 7) and nonporous Mylar membranes (n = 7) were used as positive and negative controls, respectively. Glucose concentrations were measured at 30-min intervals with a commercially available glucose meter. RESULTS: The diffusion of glucose through corneas in vitro was calculated by a simple Fickian diffusion model. The diffusion coefficient of glucose is highest for the human cornea (D(HC) = 3.0 +/- 0.2 x 10(-6) cm(2)/s) followed by porcine corneas (D(PC) = 1.8 +/- 0.6 x 10(-6) cm(2)/s) and bovine corneas (D(BC) = 1.6 +/- 0.1 x 10(-6) cm(2)/s) (p < 0.05). The diffusion coefficients of all tested corneas were significantly higher (p < 0.05) than that of dialysis membrane (D(DM) = 3.4 +/- 0.2 x 10(-7) cm(2)/s). CONCLUSION: The glucose diffusion coefficients of human, bovine, and porcine corneas are on the order of 10(-6). Human corneas have higher permeability to glucose than either porcine or bovine corneas. Copyright (c) 2006 S. Karger AG, Basel.
PURPOSE: To measure glucose flux across human, bovine, and porcine corneas and to determine the diffusion coefficient of each type of cornea. METHODS: Diffusion of glucose across human (n = 8), bovine (n = 7), and pig corneas (n = 8) was measured using a modified blind well chamber apparatus (Boyden chamber). Dialysis membranes (n = 7) and nonporous Mylar membranes (n = 7) were used as positive and negative controls, respectively. Glucose concentrations were measured at 30-min intervals with a commercially available glucose meter. RESULTS: The diffusion of glucose through corneas in vitro was calculated by a simple Fickian diffusion model. The diffusion coefficient of glucose is highest for the human cornea (D(HC) = 3.0 +/- 0.2 x 10(-6) cm(2)/s) followed by porcine corneas (D(PC) = 1.8 +/- 0.6 x 10(-6) cm(2)/s) and bovine corneas (D(BC) = 1.6 +/- 0.1 x 10(-6) cm(2)/s) (p < 0.05). The diffusion coefficients of all tested corneas were significantly higher (p < 0.05) than that of dialysis membrane (D(DM) = 3.4 +/- 0.2 x 10(-7) cm(2)/s). CONCLUSION: The glucose diffusion coefficients of human, bovine, and porcine corneas are on the order of 10(-6). Human corneas have higher permeability to glucose than either porcine or bovine corneas. Copyright (c) 2006 S. Karger AG, Basel.
Authors: Resmi A Charalel; Kristin Engberg; Jaan Noolandi; Jennifer R Cochran; Curtis Frank; Christopher N Ta Journal: Ophthalmic Res Date: 2012-03-02 Impact factor: 2.892
Authors: Mohammad Mirazul Islam; Roholah Sharifi; Shamina Mamodaly; Rakibul Islam; Daniel Nahra; Dina B Abusamra; Pui Chuen Hui; Yashar Adibnia; Mehdi Goulamaly; Eleftherios I Paschalis; Andrea Cruzat; Jing Kong; Per H Nilsson; Pablo Argüeso; Tom Eirik Mollnes; James Chodosh; Claes H Dohlman; Miguel Gonzalez-Andrades Journal: Acta Biomater Date: 2019-07-05 Impact factor: 8.947
Authors: Sina Sharifi; Hannah Sharifi; Ali Akbari; Darrell Koza; Claes H Dohlman; Eleftherios I Paschalis; James Chodosh Journal: ACS Appl Bio Mater Date: 2021-09-21
Authors: Sina Sharifi; Hannah Sharifi; Curtis Guild; Mohammad Mirazul Islam; Khoa D Tran; Corrina Patzer; Claes H Dohlman; Eleftherios I Paschalis; Miguel Gonzalez-Andrades; James Chodosh Journal: Ocul Surf Date: 2021-03-03 Impact factor: 5.033