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Literature DB >> 16397825

Intensity range based quantitative FRET data analysis to localize protein molecules in live cell nuclei.

Abstract

Förster (fluorescence) resonance energy transfer (FRET) is an ideal technique to estimate the distance between interacting protein molecules in live specimens using intensity-based microscopy. The spectral overlap of donor and acceptor- essential for FRET-also generates a contamination of the FRET signal. There are a number of algorithms available to remove this spectral bleedthrough (SBT) contamination and in this paper we compare two popular algorithms to estimate the SBT element and to calculate a more precise level of energy transfer efficiency, and with that a more accurate distance estimate.

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Year: 2006 PMID： 16397825 DOI： 10.1007/s10895-005-0024-1

Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217

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