Literature DB >> 1639394

A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene.

D A Marchuk1, R Tavakkol, M R Wallace, B H Brownstein, P Taillon-Miller, C T Fong, E Legius, L B Andersen, T W Glover, F S Collins.   

Abstract

The yeast artificial chromosome (YAC) system (Burke et al., 1987, Science 236: 806-812) allows the direct cloning of large regions of the genome. A YAC contig map of approximately 700 kb encompassing the region surrounding the type 1 neurofibromatosis (NF1) locus on 17q11.2 has been constructed. A single YAC containing the entire NF1 locus has been constructed by homologous recombination in yeast. In the process of contig construction a novel method of YAC end rescue has been developed by YAC circularization in yeast and plasmid rescue in bacteria. YACs containing homology to the NF1 region but mapping to another chromosome have also been discovered. Sequences of portions of the homologous locus indicate that this other locus is a nonprocessed pseudogene.

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Year:  1992        PMID： 1639394     DOI： 10.1016/0888-7543(92)90140-n

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  5 in total

1.  A cytogenetic deletion, del(17)(q11.22q21.1), in a patient with sporadic neurofibromatosis type 1 (NF1) associated with dysmorphism and developmental delay.

Authors:  M Upadhyaya; S H Roberts; J Maynard; E Sorour; P W Thompson; M Vaughan; A O Wilkie; H E Hughes
Journal:  J Med Genet       Date:  1996-02       Impact factor: 6.318

2.  Island rescue PCR: a rapid and efficient method for isolating transcribed sequences from yeast artificial chromosomes and cosmids.

Authors:  J M Valdes; D A Tagle; F S Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-07       Impact factor: 11.205

Review 3.  Molecular genetics of neurofibromatosis type 1 (NF1).

Authors:  M H Shen; P S Harper; M Upadhyaya
Journal:  J Med Genet       Date:  1996-01       Impact factor: 6.318

4.  A Tsp509I variant in exon 13 of the neurofibromatosis type 1 (NF1) gene allows the identification of both alleles at the mRNA level.

Authors:  V Régnier; G Danglot; V C Nguyen; A Bernheim
Journal:  Hum Genet       Date:  1995-07       Impact factor: 4.132

5.  Deletions spanning the neurofibromatosis 1 gene: identification and phenotype of five patients.

Authors:  L M Kayes; W Burke; V M Riccardi; R Bennett; P Ehrlich; A Rubenstein; K Stephens
Journal:  Am J Hum Genet       Date:  1994-03       Impact factor: 11.025
  5 in total

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