INTRODUCTION: The expansion of specific trinucleotide repeats results in certain genetic disorders. METHOD: Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. RESULT: The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C. CONCLUSION: Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
INTRODUCTION: The expansion of specific trinucleotide repeats results in certain genetic disorders. METHOD: Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene. RESULT: The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C. CONCLUSION: Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
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Authors: P M Bingham; M O Scott; S Wang; M J McPhaul; E M Wilson; J Y Garbern; D E Merry; K H Fischbeck Journal: Nat Genet Date: 1995-02 Impact factor: 38.330
Authors: K Sermon; V Goossens; S Seneca; W Lissens; A De Vos; M Vandervorst; A Van Steirteghem; I Liebaers Journal: Prenat Diagn Date: 1998-12 Impact factor: 3.050