| Literature DB >> 1639266 |
M Koizumi1, H Kamiya, E Ohtsuka.
Abstract
We have designed hammerhead ribozymes that cleave c-Ha-ras mRNA mutated at codon 12 (GGU----GUU). Plasmids containing the ribozyme-encoding genes were expressed under the control of the long terminal repeats of Rous sarcoma virus in NIH3T3 cells transfected with the activated c-Ha-ras gene. These ribozymes were found to inhibit formation of foci (by about 50%) by cleaving the oncogene mRNA, rather than by hybridizing to it. Furthermore, when the activated c-Ha-ras gene was cotransfected with the ribozyme-encoding gene, three morphologically flat colonies were found and isolated. We also found that expression of c-Ha-ras was suppressed in cells containing ribozymes.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1639266 DOI： 10.1016/0378-1119(92)90727-7
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688