Literature DB >> 16391137

Immobilization of cells with surface-displayed chitin-binding domain.

Jen-You Wang1, Yun-Peng Chao.   

Abstract

To explore chitin-binding domain (ChBD)-based cell immobilization, a tripartite gene fusion consisting of an in-frame fusion of ChBD to lpp and ompA was constructed and expressed in Escherichia coli. ChBD-displayed cells exhibited highly specific and stable binding to chitin within a wide range of pHs (5 to 8) and temperatures (15 to 37 degrees C). These results illustrate the promising use of this approach for engineering applications.

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Year:  2006        PMID： 16391137      PMCID： PMC1352180          DOI： 10.1128/AEM.72.1.927-931.2006

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

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Authors:  A A Wang; A Mulchandani; W Chen
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Journal:  Gene       Date:  1997-06-19       Impact factor: 3.688

Review 3.  Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.

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Journal:  Arch Biochem Biophys       Date:  2004-06-15       Impact factor: 4.013

5.  Improvement of the thermoregulated T7 expression system by using the heat-sensitive lacI.

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6.  Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid.

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Journal:  Macromol Biosci       Date:  2004-10-20       Impact factor: 4.979

7.  Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations.

Authors:  D A Siegele; J C Hu
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

8.  Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains.

Authors:  J A Francisco; C Stathopoulos; R A Warren; D G Kilburn; G Georgiou
Journal:  Biotechnology (N Y)       Date:  1993-04

9.  Overproduction of D-hydantoinase and carbamoylase in a soluble form in Escherichia coli.

Authors:  Y P Chao; C J Chiang; T E Lo; H Fu
Journal:  Appl Microbiol Biotechnol       Date:  2000-09       Impact factor: 4.813

10.  Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2.

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  7 in total

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4.  Nisin production in a chitin-including continuous fermentation system with Lactococcus lactis displaying a cell wall chitin-binding domain.

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5.  Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center.

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6.  Immobilization of Pichia pastoris cells containing alcohol oxidase activity.

Authors:  S Maleknia; H Ahmadi; D Norouzian
Journal:  Iran J Microbiol       Date:  2011-12

7.  Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice.

Authors:  Changye Hui; Yan Guo; Wen Zhang; Chaoxian Gao; Xueqin Yang; Yuting Chen; Limei Li; Xianqing Huang
Journal:  Sci Rep       Date:  2018-04-09       Impact factor: 4.379
  7 in total

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