BACKGROUND: Matrix metalloproteinases (MMPs) play pivotal roles in tumor progression. MMP-13 (collagenase-3) digests collagen and other extracellular components. MATERIALS AND METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and zymograph were used to study the roles of MMP-13 during the neoplastic process of oral squamous cell carcinoma (OSCC). RESULTS: Increase of MMP-13 mRNA and protein expression in OSCC cell lines relative to cultivated normal oral keratinocytes was found. MMP-13 mRNA expression in OSCC was significantly higher than in non-cancerous match tissue (NCMT) in 36 tissue pairs. Esophageal squamous cell carcinoma also exhibited high MMP-13 mRNA expression. The percentage of OSCC exhibiting strong MMP-13 immunoreactivity was significantly higher than pre-invasive lesion and NCMT. Treatment with >5 microm epigallocatechin-3-gallate (EGCG) to OEC-M1 cells suppressed the expression and activity of MMP-13. CONCLUSION: MMP-13 could be a potential tumor marker for OSCC. The effects of EGCG in tumor inhibition may act partially through the modulation of MMP-13.
BACKGROUND: Matrix metalloproteinases (MMPs) play pivotal roles in tumor progression. MMP-13 (collagenase-3) digests collagen and other extracellular components. MATERIALS AND METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and zymograph were used to study the roles of MMP-13 during the neoplastic process of oral squamous cell carcinoma (OSCC). RESULTS: Increase of MMP-13 mRNA and protein expression in OSCC cell lines relative to cultivated normal oral keratinocytes was found. MMP-13 mRNA expression in OSCC was significantly higher than in non-cancerous match tissue (NCMT) in 36 tissue pairs. Esophageal squamous cell carcinoma also exhibited high MMP-13 mRNA expression. The percentage of OSCC exhibiting strong MMP-13 immunoreactivity was significantly higher than pre-invasive lesion and NCMT. Treatment with >5 microm epigallocatechin-3-gallate (EGCG) to OEC-M1 cells suppressed the expression and activity of MMP-13. CONCLUSION:MMP-13 could be a potential tumor marker for OSCC. The effects of EGCG in tumor inhibition may act partially through the modulation of MMP-13.
Authors: Brian J Henson; Samsiddhi Bhattacharjee; Dawn M O'Dee; Eleanor Feingold; Susanne M Gollin Journal: Genes Chromosomes Cancer Date: 2009-07 Impact factor: 5.006
Authors: Georgi Mishev; Elitsa Deliverska; Ruslan Hlushchuk; Nikolay Velinov; Daniel Aebersold; Felix Weinstein; Valentin Djonov Journal: Biotechnol Biotechnol Equip Date: 2014-11-29 Impact factor: 1.632
Authors: Vui King Vincent-Chong; Iman Salahshourifar; Lee Peng Karen-Ng; Ming Yhong Siow; Thomas George Kallarakkal; Anand Ramanathan; Yi-Hsin Yang; Goot Heah Khor; Zainal Ariff Abdul Rahman; Siti Mazlipah Ismail; Narayanan Prepageran; Wan Mahadzir Wan Mustafa; Mannil Thomas Abraham; Keng Kiong Tay; Sok Ching Cheong; Rosnah Binti Zain Journal: ScientificWorldJournal Date: 2014-10-23
Authors: K Kato; N K Long; H Makita; M Toida; T Yamashita; D Hatakeyama; A Hara; H Mori; T Shibata Journal: Br J Cancer Date: 2008-07-29 Impact factor: 7.640