| Literature DB >> 16389470 |
Noriko Kotobuki1, Daisuke Kawagoe, Daishirou Nomura, Youichi Katou, Kaori Muraki, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi.
Abstract
To observe living cell morphology on ceramics by light microscopy, we fabricated a new material-transparent beta - tricalcium phosphate (t-beta TCP) ceramic-for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-beta TCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-beta TCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-beta TCP and PS substrata, indicating the excellent properties of beta TCP ceramics for BMSCs culture towards osteogenic differentiation.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16389470 DOI： 10.1007/s10856-006-6327-1
Source DB: PubMed Journal: J Mater Sci Mater Med ISSN： 0957-4530 Impact factor: 3.896