| Literature DB >> 16388307 |
Liang-Fong Wong1, Ping K Yip, Anna Battaglia, John Grist, Jonathan Corcoran, Malcolm Maden, Mimoun Azzouz, Susan M Kingsman, Alan J Kingsman, Nicholas D Mazarakis, Stephen B McMahon.
Abstract
The embryonic CNS readily undergoes regeneration, unlike the adult CNS, which has limited axonal repair after injury. Here we tested the hypothesis that retinoic acid receptor beta2 (RARbeta2), critical in development for neuronal growth, may enable adult neurons to grow in an inhibitory environment. Overexpression of RARbeta2 in adult rat dorsal root ganglion cultures increased intracellular levels of cyclic AMP and stimulated neurite outgrowth. Stable RARbeta2 expression in DRG neurons in vitro and in vivo enabled their axons to regenerate across the inhibitory dorsal root entry zone and project into the gray matter of the spinal cord. The regenerated neurons enhanced second-order neuronal activity in the spinal cord, and RARbeta2-treated rats showed highly significant improvement in sensorimotor tasks. These findings show that RARbeta2 induces axonal regeneration programs within injured neurons and may thus offer new therapeutic opportunities for CNS regeneration.Entities:
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Year: 2005 PMID： 16388307 DOI： 10.1038/nn1622
Source DB: PubMed Journal: Nat Neurosci ISSN： 1097-6256 Impact factor: 24.884