Literature DB >> 16388305

Derivation of human embryonic stem cells in defined conditions.

Tenneille E Ludwig1, Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas, James A Thomson.   

Abstract

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

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Year:  2006        PMID： 16388305     DOI： 10.1038/nbt1177

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  362 in total

1.  Distinct epigenomic landscapes of pluripotent and lineage-committed human cells.

Authors:  R David Hawkins; Gary C Hon; Leonard K Lee; Queminh Ngo; Ryan Lister; Mattia Pelizzola; Lee E Edsall; Samantha Kuan; Ying Luu; Sarit Klugman; Jessica Antosiewicz-Bourget; Zhen Ye; Celso Espinoza; Saurabh Agarwahl; Li Shen; Victor Ruotti; Wei Wang; Ron Stewart; James A Thomson; Joseph R Ecker; Bing Ren
Journal:  Cell Stem Cell       Date:  2010-05-07       Impact factor: 24.633

Review 2.  Cryopreservation of human pluripotent stem cells in defined medium.

Authors:  Weiwei Liu; Guokai Chen
Journal:  Curr Protoc Stem Cell Biol       Date:  2014-11-03

3.  Effects of ionizing radiation on self-renewal and pluripotency of human embryonic stem cells.

Authors:  Kitchener D Wilson; Ning Sun; Mei Huang; Wendy Y Zhang; Andrew S Lee; Zongjin Li; Shan X Wang; Joseph C Wu
Journal:  Cancer Res       Date:  2010-06-08       Impact factor: 12.701

Review 4.  Regenerative chemical biology: current challenges and future potential.

Authors:  Ada Ao; Jijun Hao; Charles C Hong
Journal:  Chem Biol       Date:  2011-04-22

Review 5.  Using human pluripotent stem cells to untangle neurodegenerative disease mechanisms.

Authors:  Brigitte Malgrange; Laurence Borgs; Benjamin Grobarczyk; Audrey Purnelle; Patricia Ernst; Gustave Moonen; Laurent Nguyen
Journal:  Cell Mol Life Sci       Date:  2010-10-26       Impact factor: 9.261

6.  Microfibrous substrate geometry as a critical trigger for organization, self-renewal, and differentiation of human embryonic stem cells within synthetic 3-dimensional microenvironments.

Authors:  Aaron L Carlson; Charles A Florek; Joseph J Kim; Thomas Neubauer; Jennifer C Moore; Rick I Cohen; Joachim Kohn; Martin Grumet; Prabhas V Moghe
Journal:  FASEB J       Date:  2012-04-27       Impact factor: 5.191

7.  Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.

Authors:  Veronica Sanchez-Freire; Antje D Ebert; Tomer Kalisky; Stephen R Quake; Joseph C Wu
Journal:  Nat Protoc       Date:  2012-04-05       Impact factor: 13.491

8.  Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling.

Authors:  Linlin Wang; Thomas C Schulz; Eric S Sherrer; Derek S Dauphin; Soojung Shin; Angelique M Nelson; Carol B Ware; Mei Zhan; Chao-Zhong Song; Xiaoji Chen; Sandii N Brimble; Amanda McLean; Maria J Galeano; Elizabeth W Uhl; Kevin A D'Amour; Jonathan D Chesnut; Mahendra S Rao; C Anthony Blau; Allan J Robins
Journal:  Blood       Date:  2007-08-29       Impact factor: 22.113

9.  ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells.

Authors:  David A Claassen; Michelle M Desler; Angie Rizzino
Journal:  Mol Reprod Dev       Date:  2009-08       Impact factor: 2.609

10.  Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.

Authors:  Jeanette Beers; Daniel R Gulbranson; Nicole George; Lauren I Siniscalchi; Jeffrey Jones; James A Thomson; Guokai Chen
Journal:  Nat Protoc       Date:  2012-10-25       Impact factor: 13.491
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