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Literature DB >> 16382334

Time-resolved fluorescence studies on bovine serum albumin denaturation process.

Abstract

The denaturation of Bovine Serum Albumin (BSA) by a chaotropic agent, guanidinium hydrochloride (GuH+Cl-) was studied by fluorescence lifetime analysis. The BSA was labelled with 1-anilino-8-naphthalene sulfonate (ANS) at two different molar ratios (1:1) and (1:10). The non-exponential fluorescence kinetics of the BSA-ANS complex at different stages of denaturation is analysed using three different models: a discrete tri-exponential sum, stretched exponential, and Gaussian lifetime distribution. In all cases, the fluorescence decay times decreased with protein denaturation. The results from the models show that there are at least two different binding sites located in the BSA protein with different water accessibility.

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Year: 2005 PMID： 16382334 DOI： 10.1007/s10895-005-0029-9

Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217

15 in total

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7 in total

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