Literature DB >> 16381658

A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics.

Hyeon-Jin Sun1, Sayaka Uchii, Shin Watanabe, Hiroshi Ezura.   

Abstract

We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16381658     DOI： 10.1093/pcp/pci251

Source DB:  PubMed          Journal:  Plant Cell Physiol        ISSN： 0032-0781            Impact factor:   4.927


  98 in total

Review 1.  From miracle fruit to transgenic tomato: mass production of the taste-modifying protein miraculin in transgenic plants.

Authors:  Kyoko Hiwasa-Tanase; Tadayoshi Hirai; Kazuhisa Kato; Narendra Duhita; Hiroshi Ezura
Journal:  Plant Cell Rep       Date:  2011-12-08       Impact factor: 4.570

2.  PL1 fusion gene: a novel visual selectable marker gene that confers tolerance to multiple abiotic stresses in transgenic tomato.

Authors:  Feng Jin; Shu Li; Lijie Dang; Wenting Chai; Pengli Li; Ning Ning Wang
Journal:  Transgenic Res       Date:  2012-10       Impact factor: 2.788

3.  Novel promoters that induce specific transgene expression during the green to ripening stages of tomato fruit development.

Authors:  Kyoko Hiwasa-Tanase; Hirofumi Kuroda; Tadayoshi Hirai; Koh Aoki; Kenichi Takane; Hiroshi Ezura
Journal:  Plant Cell Rep       Date:  2012-04-06       Impact factor: 4.570

4.  Expression analysis of the auxin efflux carrier family in tomato fruit development.

Authors:  Sogo Nishio; Ryo Moriguchi; Hiroki Ikeda; Hideki Takahashi; Hideyuki Takahashi; Nobuharu Fujii; Thomas J Guilfoyle; Koki Kanahama; Yoshinori Kanayama
Journal:  Planta       Date:  2010-06-23       Impact factor: 4.116

5.  High-level accumulation of recombinant miraculin protein in transgenic tomatoes expressing a synthetic miraculin gene with optimized codon usage terminated by the native miraculin terminator.

Authors:  Kyoko Hiwasa-Tanase; Mpanja Nyarubona; Tadayoshi Hirai; Kazuhisa Kato; Takanari Ichikawa; Hiroshi Ezura
Journal:  Plant Cell Rep       Date:  2010-11-13       Impact factor: 4.570

6.  Suppression of the D-class MADS-box AGL11 gene triggers seedlessness in fleshy fruits.

Authors:  Nallatt Ocarez; Nilo Mejía
Journal:  Plant Cell Rep       Date:  2015-11-13       Impact factor: 4.570

7.  Small and remarkable: The Micro-Tom model system as a tool to discover novel hormonal functions and interactions.

Authors:  Marcelo Lattarulo Campos; Rogério Falleiros Carvalho; Vagner Augusto Benedito; Lázaro Eustáquio Pereira Peres
Journal:  Plant Signal Behav       Date:  2010-03-12

8.  Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.

Authors:  Tadayoshi Hirai; You-Wang Kim; Kazuhisa Kato; Kyoko Hiwasa-Tanase; Hiroshi Ezura
Journal:  Transgenic Res       Date:  2011-02-27       Impact factor: 2.788

9.  Evaluation of four Agrobacterium tumefaciens strains for the genetic transformation of tomato (Solanum lycopersicum L.) cultivar Micro-Tom.

Authors:  V J Chetty; N Ceballos; D Garcia; J Narváez-Vásquez; W Lopez; M L Orozco-Cárdenas
Journal:  Plant Cell Rep       Date:  2012-10-26       Impact factor: 4.570

10.  Comprehensive resources for tomato functional genomics based on the miniature model tomato micro-tom.

Authors:  C Matsukura; K Aoki; N Fukuda; T Mizoguchi; E Asamizu; T Saito; D Shibata; H Ezura
Journal:  Curr Genomics       Date:  2008-11       Impact factor: 2.236
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.