| Literature DB >> 16378731 |
Rukhsana Sultana1, H Fai Poon, Jian Cai, William M Pierce, Michael Merchant, Jon B Klein, William R Markesbery, D Allan Butterfield.
Abstract
Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3-phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid beta-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.Entities:
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Year: 2005 PMID： 16378731 DOI： 10.1016/j.nbd.2005.10.004
Source DB: PubMed Journal: Neurobiol Dis ISSN： 0969-9961 Impact factor: 5.996