Literature DB >> 1637332

A member of the eukaryotic subtilisin family (PC3) has the enzymic properties of the type 1 proinsulin-converting endopeptidase.

E M Bailyes1, K I Shennan, A J Seal, S P Smeekens, D F Steiner, J C Hutton, K Docherty.   

Abstract

PC3, a mammalian homologue of the yeast subtilisin-like proteinase Kex2, was expressed in Xenopus oocytes and its activity was characterized. PC3 cleaved human proinsulin at one of the two dibasic sites (KTRR32 but not LQKR65). The specificity, inhibitor profile, pH optimum (5.5) and Ca(2+)-dependence (K0.5 = 2.5-3 mM) paralleled those of the insulin-granule type 1 endopeptidase activity, suggesting a role for PC3 in the conversion of prohormones.

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Year:  1992        PMID： 1637332      PMCID： PMC1132800          DOI： 10.1042/bj2850391

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Prohormone convertases revealed at last.

Authors:  D F Steiner
Journal:  Curr Biol       Date:  1991-12       Impact factor: 10.834

Review 2.  Subtilisin-like proteinases involved in the activation of proproteins of the eukaryotic secretory pathway.

Authors:  J C Hutton
Journal:  Curr Opin Cell Biol       Date:  1990-12       Impact factor: 8.382

3.  Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes.

Authors:  L Thomas; R Leduc; B A Thorne; S P Smeekens; D F Steiner; G Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

4.  Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.

Authors:  J Korner; J Chun; D Harter; R Axel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

5.  Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.

Authors:  S P Smeekens; A S Avruch; J LaMendola; S J Chan; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

6.  Characterization of PC2, a mammalian Kex2 homologue, following expression of the cDNA in microinjected Xenopus oocytes.

Authors:  K I Shennan; S P Smeekens; D F Steiner; K Docherty
Journal:  FEBS Lett       Date:  1991-06-24       Impact factor: 4.124

7.  Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes.

Authors:  W J van de Ven; J Voorberg; R Fontijn; H Pannekoek; A M van den Ouweland; H L van Duijnhoven; A J Roebroek; R J Siezen
Journal:  Mol Biol Rep       Date:  1990-11       Impact factor: 2.316

8.  Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2.

Authors:  N G Seidah; M Marcinkiewicz; S Benjannet; L Gaspar; G Beaubien; M G Mattei; C Lazure; M Mbikay; M Chrétien
Journal:  Mol Endocrinol       Date:  1991-01

9.  Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway.

Authors:  M Hosaka; M Nagahama; W S Kim; T Watanabe; K Hatsuzawa; J Ikemizu; K Murakami; K Nakayama
Journal:  J Biol Chem       Date:  1991-07-05       Impact factor: 5.157

10.  Site-directed mutagenesis and expression of PC2 in microinjected Xenopus oocytes.

Authors:  K I Shennan; A J Seal; S P Smeekens; D F Steiner; K Docherty
Journal:  J Biol Chem       Date:  1991-12-15       Impact factor: 5.157
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  35 in total

1.  Measurement of secretory vesicle pH reveals intravesicular alkalinization by vesicular monoamine transporter type 2 resulting in inhibition of prohormone cleavage.

Authors:  C G Blackmore; A Varro; R Dimaline; L Bishop; D V Gallacher; G J Dockray
Journal:  J Physiol       Date:  2001-03-15       Impact factor: 5.182

2.  Endoplasmic reticulum Ca2+ is important for the proteolytic processing and intracellular transport of proinsulin in the pancreatic beta-cell.

Authors:  P C Guest; E M Bailyes; J C Hutton
Journal:  Biochem J       Date:  1997-04-15       Impact factor: 3.857

3.  Proinsulin processing in the rat insulinoma cell line INS after overexpression of the endoproteases PC2 or PC3 by recombinant adenovirus.

Authors:  J C Irminger; K Meyer; P Halban
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

4.  Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic β cells.

Authors:  Chong Wee Liew; Anke Assmann; Andrew T Templin; Jeffrey C Raum; Kathryn L Lipson; Sindhu Rajan; Guifen Qiang; Jiang Hu; Dan Kawamori; Iris Lindberg; Louis H Philipson; Nahum Sonenberg; Allison B Goldfine; Doris A Stoffers; Raghavendra G Mirmira; Fumihiko Urano; Rohit N Kulkarni
Journal:  Proc Natl Acad Sci U S A       Date:  2014-05-19       Impact factor: 11.205

5.  Regulation of pancreatic PC1 and PC2 associated with increased glucagon-like peptide 1 in diabetic rats.

Authors:  Y Nie; M Nakashima; P L Brubaker; Q L Li; R Perfetti; E Jansen; Y Zambre; D Pipeleers; T C Friedman
Journal:  J Clin Invest       Date:  2000-04       Impact factor: 14.808

6.  Amine precursor uptake and decarboxylation: significance for processing of the rat gastrin precursor.

Authors:  S Voronina; J Henry; C Vaillant; G J Dockray; A Varro
Journal:  J Physiol       Date:  1997-06-01       Impact factor: 5.182

7.  Insulinoma with hyperproinsulinemia during hypoglycemia and loss of expression of vacuolar-type H(+)-ATPase (V-ATPase) in the tumor tissue.

Authors:  A Hiura; E C Kim; T Ikahara; K Mishima; K Shindo; T Ohta; K Satake
Journal:  Int J Pancreatol       Date:  1999-02

Review 8.  The alphaviruses: gene expression, replication, and evolution.

Authors:  J H Strauss; E G Strauss
Journal:  Microbiol Rev       Date:  1994-09

9.  Levels of the conversion endoproteases PC1 (PC3) and PC2 distinguish between insulin-producing pancreatic islet beta cells and non-beta cells.

Authors:  M Neerman-Arbez; V Cirulli; P A Halban
Journal:  Biochem J       Date:  1994-05-15       Impact factor: 3.857

10.  Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3.

Authors:  S P Smeekens; A G Montag; G Thomas; C Albiges-Rizo; R Carroll; M Benig; L A Phillips; S Martin; S Ohagi; P Gardner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205
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