Literature DB >> 1637323

A simple, fast, one-step method for the purification of the skeletal-muscle ryanodine receptor.

V Shoshan-Barmatz1, A Zarka.   

Abstract

In this paper we describe a simple, fast, one-step method for the purification of the skeletal-muscle ryanodine receptor. The ryanodine receptor from CHAPS-solubilized junctional sarcoplasmic-reticulum membranes was adsorbed to a spermine-agarose column and eluted by 2 mM-spermine. The purified receptor, consisting predominantly of a 450 kDa polypeptide on SDS/PAGE, binds [3H]ryanodine with a specific activity of approximately 300 pmol/mg of protein and with a high affinity (KD = 4.7 +/- 2 nM). The purified receptor appears to retain the pharmacological properties of the receptor in the original membranes. The purification resulted in over 80% recovery of the initial ryanodine-binding sites and about 30-96-fold purification. This simple and fast method is highly reproducible and suitable for purification of small as well as large quantities of ryanodine receptor.

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Year:  1992        PMID： 1637323      PMCID： PMC1132744          DOI： 10.1042/bj2850061

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

1.  A procedure for purification of the ryanodine receptor from skeletal muscle.

Authors:  M J Hawkes; M Díaz-Muñoz; S L Hamilton
Journal:  Membr Biochem       Date:  1989

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Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 3.  The muscle ryanodine receptor and its intrinsic Ca2+ channel activity.

Authors:  F A Lai; G Meissner
Journal:  J Bioenerg Biomembr       Date:  1989-04       Impact factor: 2.945

4.  Cardiac ryanodine receptor is absent in type I slow skeletal muscle fibers: immunochemical and ryanodine binding studies.

Authors:  T Imagawa; T Takasago; M Shigekawa
Journal:  J Biochem       Date:  1989-08       Impact factor: 3.387

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Authors:  F A Lai; H P Erickson; E Rousseau; Q Y Liu; G Meissner
Journal:  Nature       Date:  1988-01-28       Impact factor: 49.962

6.  Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor.

Authors:  H Takeshima; S Nishimura; T Matsumoto; H Ishida; K Kangawa; N Minamino; H Matsuo; M Ueda; M Hanaoka; T Hirose
Journal:  Nature       Date:  1989-06-08       Impact factor: 49.962

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Positive cooperativity of ryanodine binding to the calcium release channel of sarcoplasmic reticulum from heart and skeletal muscle.

Authors:  S G McGrew; C Wolleben; P Siegl; M Inui; S Fleischer
Journal:  Biochemistry       Date:  1989-02-21       Impact factor: 3.162

9.  The ryanodine receptor-Ca2+ release channel complex of skeletal muscle sarcoplasmic reticulum. Evidence for a cooperatively coupled, negatively charged homotetramer.

Authors:  F A Lai; M Misra; L Xu; H A Smith; G Meissner
Journal:  J Biol Chem       Date:  1989-10-05       Impact factor: 5.157

10.  Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel.

Authors:  K P Campbell; C M Knudson; T Imagawa; A T Leung; J L Sutko; S D Kahl; C R Raab; L Madson
Journal:  J Biol Chem       Date:  1987-05-15       Impact factor: 5.157
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  7 in total

1.  Modification of ryanodine receptor/Ca2+ release channel with dinitrofluorobenzene.

Authors:  N Hadad; W Feng; V Shoshan-Barmatz
Journal:  Biochem J       Date:  1999-08-15       Impact factor: 3.857

2.  Calexcitin interaction with neuronal ryanodine receptors.

Authors:  T J Nelson; W Q Zhao; S Yuan; A Favit; L Pozzo-Miller; D L Alkon
Journal:  Biochem J       Date:  1999-07-15       Impact factor: 3.857

3.  Endogenous, Ca(2+)-dependent cysteine-protease cleaves specifically the ryanodine receptor/Ca2+ release channel in skeletal muscle.

Authors:  V Shoshan-Barmatz; S Weil; H Meyer; M Varsanyi; L M Heilmeyer
Journal:  J Membr Biol       Date:  1994-12       Impact factor: 1.843

4.  Diethyl pyrocarbonate modification of the ryanodine receptor/Ca2+ channel from skeletal muscle.

Authors:  V Shoshan-Barmatz; S Weil
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857

5.  The interaction of local anesthetics with the ryanodine receptor of the sarcoplasmic reticulum.

Authors:  V Shoshan-Barmatz; S Zchut
Journal:  J Membr Biol       Date:  1993-04       Impact factor: 1.843

6.  Ryanodine receptor/calcium release channel conformations as reflected in the different effects of propranolol on its ryanodine binding and channel activity.

Authors:  S Zchut; W Feng; V Shoshan-Barmatz
Journal:  Biochem J       Date:  1996-04-15       Impact factor: 3.857

7.  Amino acid residues 4425-4621 localized on the three-dimensional structure of the skeletal muscle ryanodine receptor.

Authors:  B L Benacquista; M R Sharma; M Samsó; F Zorzato; S Treves; T Wagenknecht
Journal:  Biophys J       Date:  2000-03       Impact factor: 4.033
  7 in total

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