Literature DB >> 16371239

Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene.

Inge Vliegen1, Jan A Jacobs, Erik Beuken, Cathrien A Bruggeman, Cornelis Vink.   

Abstract

To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h.

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Year:  2005        PMID： 16371239     DOI： 10.1016/j.mimet.2005.11.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  10 in total

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Authors:  Sheng-Chuan Hsi; Jun-Ren Sun; Tzong-Shi Chiueh
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2.  Role of the siderophore transporter SirABC in the Staphylococcus aureus resistance to oxidative stress.

Authors:  Lígia S Nobre; Lígia M Saraiva
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3.  Identification of a variety of Staphylococcus species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

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Journal:  J Clin Microbiol       Date:  2009-12-23       Impact factor: 5.948

4.  One-day workflow scheme for bacterial pathogen detection and antimicrobial resistance testing from blood cultures.

Authors:  Wendy L J Hansen; Judith Beuving; Annelies Verbon; Petra F G Wolffs
Journal:  J Vis Exp       Date:  2012-07-09       Impact factor: 1.355

5.  Antibiotic susceptibility testing of grown blood cultures by combining culture and real-time polymerase chain reaction is rapid and effective.

Authors:  Judith Beuving; Annelies Verbon; Firza A Gronthoud; Ellen E Stobberingh; Petra F G Wolffs
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6.  Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites.

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7.  Automated 16S Sequencing Using an R-Based Analysis Module for Bacterial Identification.

Authors:  Kerstin Locher; Corrie R Belanger; Eric Eckbo; Melissa Caza; Billie Velapatino; Marthe K Charles
Journal:  Microbiol Spectr       Date:  2022-04-11

8.  Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung.

Authors:  Melissa Doud; Erliang Zeng; Lisa Schneper; Giri Narasimhan; Kalai Mathee
Journal:  Hum Genomics       Date:  2009-04       Impact factor: 4.639

9.  Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens.

Authors:  Linxiang Chen; Ying Cai; Guangbiao Zhou; Xiaojun Shi; Jianhui Su; Guanwu Chen; Kun Lin
Journal:  PLoS One       Date:  2014-02-14       Impact factor: 3.240

10.  High rates of antimicrobial drug resistance gene acquisition after international travel, The Netherlands.

Authors:  Christian J H von Wintersdorff; John Penders; Ellen E Stobberingh; Astrid M L Oude Lashof; Christian J P A Hoebe; Paul H M Savelkoul; Petra F G Wolffs
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  10 in total

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