Identification and partial characterization of the salmon calcitonin/CGRP gene by polymerase chain reaction.

The polymerase chain reaction was used to amplify sequences encoding calcitonin and CGRP in the genomic DNA of salmon. Amplification products of the expected length were cloned and sequenced. In this way a new CGRP-coding sequence was identified. The new sequence and the known salmon calcitonin-coding sequence were shown to be part of one gene, implying that alternative gene expression takes place in fish.