Literature DB >> 16369876

Extremely rapid extraction of DNA from bacteria and yeasts.

Hai-Rong Cheng1, Ning Jiang.   

Abstract

A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from bacteria and yeasts.

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Year:  2006        PMID： 16369876     DOI： 10.1007/s10529-005-4688-z

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  75 in total

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Journal:  J Bacteriol       Date:  2012-03-09       Impact factor: 3.490

2.  Cloning, expression, and characterization of a thermostable glucose-6-phosphate dehydrogenase from Thermoanaerobacter tengcongensis.

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3.  Characterization of an α-l-fucosidase from the periodontal pathogen Tannerella forsythia.

Authors:  Z A Megson; A Koerdt; H Schuster; R Ludwig; B Janesch; A Frey; K Naylor; I B H Wilson; G P Stafford; P Messner; C Schäffer
Journal:  Virulence       Date:  2015       Impact factor: 5.882

4.  Pathogenic Providencia alcalifaciens strain that causes fatal hemorrhagic pneumonia in piglets.

Authors:  Xinglong Wang; Jinqiu Wang; Huafang Hao; Li Qiu; Haijing Liu; Shengli Chen; Ruyi Dang; Zengqi Yang
Journal:  Curr Microbiol       Date:  2013-10-16       Impact factor: 2.188

5.  Green Extracellular Synthesis of the Silver Nanoparticles Using Thermophilic Bacillus Sp. AZ1 and its Antimicrobial Activity Against Several Human Pathogenetic Bacteria.

Authors:  Ali Deljou; Samad Goudarzi
Journal:  Iran J Biotechnol       Date:  2016-06       Impact factor: 1.671

6.  In silico analysis, molecular cloning, expression and characterization of l-asparaginase gene from Lactobacillus reuteri DSM 20016.

Authors:  Suresh Susan Aishwarya; Sellamuthu Iyappan; Kamepali Vijaya Lakshmi; Kandathil Narayanan Rajnish
Journal:  3 Biotech       Date:  2017-09-25       Impact factor: 2.406

7.  Production, characterization and antimicrobial activities of bio-pigments by Aquisalibacillus elongatus MB592, Salinicoccus sesuvii MB597, and Halomonas aquamarina MB598 isolated from Khewra Salt Range, Pakistan.

Authors:  Anila Fariq; Azra Yasmin; Muhammad Jamil
Journal:  Extremophiles       Date:  2019-05-07       Impact factor: 2.395

8.  In vitro Ca(2+)-dependent maturation of milk-clotting recombinant Epr: minor extracellular protease: from Bacillus licheniformis.

Authors:  José Manuel Ageitos; Juan Andrés Vallejo; Manuel Serrat; Angeles Sánchez-Pérez; Tomás G Villa
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695

Review 9.  Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis.

Authors:  Scott Quainoo; Jordy P M Coolen; Sacha A F T van Hijum; Martijn A Huynen; Willem J G Melchers; Willem van Schaik; Heiman F L Wertheim
Journal:  Clin Microbiol Rev       Date:  2017-10       Impact factor: 26.132

10.  Cloning, purification and characterization of an NAD-Dependent D-Arabitol dehydrogenase from acetic acid bacterium, Acetobacter suboxydans.

Authors:  Hairong Cheng; Zilong Li; Ning Jiang; Zixin Deng
Journal:  Protein J       Date:  2009-08       Impact factor: 2.371
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