Artificial neural networks in bioprocess state estimation.

The application of artificial neural networks to the estimation and prediction of bioprocess variables is presented in this paper. A neural network methodology is discussed, which uses environmental and physiological information available from on-line sensors, to estimate concentration of species in the bioreactor. Two case studies are presented, both based on the ethanol production by Zymomonas mobilis. An efficient optimization algorithm which reduces the number of iterations required for convergence is proposed. Results are presented for different training sets and different training methodologies. It is shown that the neural network estimator provides good on-line bioprocess state estimations.