| Literature DB >> 16364484 |
Madhavan Nallani1, Samantha Benito, Ozana Onaca, Alexandra Graff, Marcus Lindemann, Mathias Winterhalter, Wolfgang Meier, Ulrich Schwaneberg.
Abstract
A nanocompartment system based on two deletion mutants of the large channel protein FhuA (FhuA Delta1-129; FhuA Delta1-160) and an ABA triblock copolymer (PMOXA-PDMS-PMOXA) has been developed for putative biotechnological applications. FhuA is ideally suited for applications in biotechnology due to its monomeric structure, large pore diameter (39-46 A elliptical cross-section) that ensures rapid compound flux, and solved crystallographic structure. Two areas of application were targeted as proof of principle: (A) selective product recovery in nanocompartments and (B) enzymatic conversion in nanocompartments. Selective recovery of negatively charged compounds has been achieved on the example of sulforhodamine B by using positively charged polylysine molecules as trap inside the nanocompartment. Conversion in nanocompartments has been achieved by 3,3',5,5'-tetramethylbenzidine oxidation employing horseradish peroxidase (HRP).Entities:
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Year: 2005 PMID： 16364484 DOI： 10.1016/j.jbiotec.2005.10.025
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307