| Literature DB >> 16364342 |
Xiao Wang1, Yanling Geng, Fuwei Li, Qianshan Gao, Xingang Shi.
Abstract
pH-zone-refining counter-current chromatography was successfully applied to the separation of cichoric acid from Echinacea Purpurea (L.) Moench. A 3.0 g quantity of sample was separated using the following two-phase solvent system: MtBE-CH3CN-water (4:1:5, v/v), 10 mM trifluoroacetic acid in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and negative ion electrospray ionization mass spectrometry. Double separations were performed with the same solvent system yielding 563 mg cichoric acid at 95.6% purity.Entities:
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Year: 2005 PMID： 16364342 DOI： 10.1016/j.chroma.2005.11.092
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759