| Literature DB >> 16356828 |
Li-Jiuan Shen1, Karin Beloussow, Wei-Chiang Shen.
Abstract
Arginine deiminase (ADI), currently in clinical trials, has various biological activities including anti-proliferation, anti-angiogenesis and inhibition of inducible nitric oxide synthase (iNOS). To recognize limitations and therapeutic applications, the mechanism of ADI modulation of arginine metabolic pathways was investigated. MCF-7 and A549 cells have notable different sensitivity to recombinant ADI (rADI) and express diverse argininosuccinate synthase (AS) activity, which regenerates arginine. Due to compartmentalization of arginine, utilization of arginine for protein synthesis occurs from either the intracellular arginine pool or the citrulline-arginine-regeneration pathway, whereas for polyamine synthesis, utilization is only from the intracellular arginine pool. Modulating AS activity or introducing rADI intracellularly to reduce intracellular arginine regeneration may expand therapeutic applications of rADI.Entities:
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Year: 2006 PMID： 16356828 DOI： 10.1016/j.canlet.2005.01.007
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679