PURPOSE: Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in human donor eyes and to examine the effect of human MMP-3 transgene expression in a rat model in vivo. METHODS: A viscocanalostomy-like operation was performed and adenoviral vector encoding for MMP-3 and green fluorescent protein was injected into human Schlemm canal or rat anterior chamber. RESULTS: Transgene expression was high in trabecular meshwork endothelium in human donor eyes. In vivo, adenovirus caused dose-dependent inflammation. CONCLUSIONS: Direct injection of adenoviral vectors into the Schlemm canal has potential in glaucoma treatment.
PURPOSE: Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in humandonor eyes and to examine the effect of humanMMP-3 transgene expression in a rat model in vivo. METHODS: A viscocanalostomy-like operation was performed and adenoviral vector encoding for MMP-3 and green fluorescent protein was injected into human Schlemm canal or rat anterior chamber. RESULTS: Transgene expression was high in trabecular meshwork endothelium in humandonor eyes. In vivo, adenovirus caused dose-dependent inflammation. CONCLUSIONS: Direct injection of adenoviral vectors into the Schlemm canal has potential in glaucoma treatment.
Authors: Eun Suk Lee; Carol A Rasmussen; Mark S Filla; Sarah R Slauson; Aaron W Kolb; Donna M Peters; Paul L Kaufman; B'Ann T Gabelt; Curtis R Brandt Journal: Curr Eye Res Date: 2014-02-21 Impact factor: 2.424
Authors: Xuyang Liu; Carol A Rasmussen; B'ann T Gabelt; Curtis R Brandt; Paul L Kaufman Journal: Surv Ophthalmol Date: 2009 Jul-Aug Impact factor: 6.048
Authors: Zeynep Aktas; Baohe Tian; Jared McDonald; Ron Yamamato; Christine Larsen; Julie Kiland; Paul L Kaufman; Carol A Rasmussen Journal: J Ocul Pharmacol Ther Date: 2014-02-10 Impact factor: 2.671