J D Tankson1, P J Fedorka-Cray, C R Jackson, M Headrick. 1. USDA/Agricultural Research Services, Bacterial and Epidemiology Antimicrobial Resistance Research Unit, 950 College Station Road, Athens, GA 30605, USA.
Abstract
BACKGROUND: In the United States, Salmonella enterica serotype Niakhar is infrequently isolated. Between 1997 and 2000, the animal arm of the National Antimicrobial Resistance Monitoring System-Enteric Bacteria (NARMS) assayed a total of 22,383 Salmonella isolates from various animal sources (swine, cattle, chickens, turkeys, cats, horses, exotics and dogs) for antimicrobial susceptibility. Isolates originated from diagnostic and non-diagnostic submissions. OBJECTIVES: To study the phenotypic and genotypic characteristics of Salmonella Niakhar. METHODS AND RESULTS: Only five (0.02%) of the 22,383 isolates were identified as Salmonella Niakhar. Antimicrobial resistance testing indicated that three isolates were pan-susceptible, one isolate was resistant to ampicillin and one isolate was resistant to ampicillin, chloramphenicol, ciprofloxacin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline and trimethoprim/sulfamethoxazole. RAPD-PCR analysis, PFGE and ribotyping indicated that two pan-susceptible isolates were genetically similar, whereas the three remaining isolates were genetically different. The one Salmonella Niakhar isolate that was multiresistant harboured a class I integron, intI1 and two large plasmids. CONCLUSIONS: This study represents the first report of a ciprofloxacin-resistant Salmonella isolate from the animal arm of NARMS.
BACKGROUND: In the United States, Salmonella enterica serotype Niakhar is infrequently isolated. Between 1997 and 2000, the animal arm of the National Antimicrobial Resistance Monitoring System-Enteric Bacteria (NARMS) assayed a total of 22,383 Salmonella isolates from various animal sources (swine, cattle, chickens, turkeys, cats, horses, exotics and dogs) for antimicrobial susceptibility. Isolates originated from diagnostic and non-diagnostic submissions. OBJECTIVES: To study the phenotypic and genotypic characteristics of Salmonella Niakhar. METHODS AND RESULTS: Only five (0.02%) of the 22,383 isolates were identified as Salmonella Niakhar. Antimicrobial resistance testing indicated that three isolates were pan-susceptible, one isolate was resistant to ampicillin and one isolate was resistant to ampicillin, chloramphenicol, ciprofloxacin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline and trimethoprim/sulfamethoxazole. RAPD-PCR analysis, PFGE and ribotyping indicated that two pan-susceptible isolates were genetically similar, whereas the three remaining isolates were genetically different. The one Salmonella Niakhar isolate that was multiresistant harboured a class I integron, intI1 and two large plasmids. CONCLUSIONS: This study represents the first report of a ciprofloxacin-resistant Salmonella isolate from the animal arm of NARMS.
Authors: Sohyun Cho; Lari M Hiott; Sandra L House; Tiffanie A Woodley; Elizabeth A McMillan; Poonam Sharma; John B Barrett; Eric S Adams; Joshua M Brandenburg; Kelley B Hise; Jacob M Bateman McDonald; Elizabeth A Ottesen; Erin K Lipp; Charlene R Jackson; Jonathan G Frye Journal: Appl Environ Microbiol Date: 2022-05-09 Impact factor: 5.005
Authors: Sohyun Cho; Hoang Anh Thi Nguyen; Jacob M McDonald; Tiffanie A Woodley; Lari M Hiott; John B Barrett; Charlene R Jackson; Jonathan G Frye Journal: Int J Environ Res Public Health Date: 2019-10-07 Impact factor: 3.390