| Literature DB >> 16350404 |
Margaret E McLaughlin-Drubin1, Craig Meyers.
Abstract
The organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including virion morphogenesis. This system utilizes HPV-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with HPV genomic DNA. When grown as raft cultures, these lines allow for a detailed study of all stages of the viral life cycle. In this chapter, we describe in detail how to (1) culture keratinocytes, (2) electroporate primary keratinocytes with HPV DNA, (3) detect episomal HPV genomes by Southern (DNA) blotting, (4) grow organotypic raft cultures, (5) isolate HPV, and (6) perform in vitro infectivity testing.Entities:
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Year: 2005 PMID： 16350404 DOI： 10.1385/1-59259-982-6:171
Source DB: PubMed Journal: Methods Mol Med ISSN： 1543-1894