Literature DB >> 16349935

Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.

A L van Wezel1, P A van Hemert, W Parisius, N B Cucakovich, H G Macmorine.   

Abstract

Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.

Entities:  

Year:  1972        PMID： 16349935      PMCID： PMC376550          DOI： 10.1128/am.24.3.506-507.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  4 in total

1.  An apparatus for producing trypsinized monkey kidney cell suspensions.

Authors:  L W BISHOP; M K SMITH; A J BEALE
Journal:  Virology       Date:  1960-02       Impact factor: 3.616

2.  Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions.

Authors:  C RAPPAPORT
Journal:  Bull World Health Organ       Date:  1956       Impact factor: 9.408

3.  Poliomyelitis viruses in tissue culture. IV. Protein-free nutrient media in stationary and roller tube cultures.

Authors:  J L MELNICK; J T RIORDAN
Journal:  Proc Soc Exp Biol Med       Date:  1952-10

4.  Plaque formation and isolation of pure lines with poliomyelitis viruses.

Authors:  R DULBECCO; M VOGT
Journal:  J Exp Med       Date:  1954-02       Impact factor: 14.307
  4 in total
  1 in total

1.  An immunofluorescence study of influenza virus filament formation.

Authors:  J C Cox; A W Hampson; R C Hamilton
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574
  1 in total

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