Literature DB >> 16349929

Demonstration of rhodanese activity in polyacrylamide gels.

J R Murphy1, J Frankenfeld, J Calvert, R C Tilton.   

Abstract

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

Entities:  

Year:  1972        PMID： 16349929      PMCID： PMC380596          DOI： 10.1128/am.24.2.283-285.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels.

Authors:  R L HUNTER; C L MARKERT
Journal:  Science       Date:  1957-06-28       Impact factor: 47.728

2.  Procedure for rapid and sensitive detection of rhodanese separated by polyacrylamide gel electrophoresis.

Authors:  G G Guilbault; S S Kuan; R Cochran
Journal:  Anal Biochem       Date:  1971-09       Impact factor: 3.365

3.  Starch gel electrophoresis of enzymes--a compilation of recipes.

Authors:  C R Shaw; R Prasad
Journal:  Biochem Genet       Date:  1970-04       Impact factor: 1.890

4.  Survey of the photosynthetic bacteria for rhodanese (thiosulfate: cyanide sulfur transferase) activity.

Authors:  D C Yoch; E S Lindstrom
Journal:  J Bacteriol       Date:  1971-05       Impact factor: 3.490

5.  Observations on the rhodanese activity of Desulfotomaculum nigrificans.

Authors:  C P Burton; J M Akagi
Journal:  J Bacteriol       Date:  1971-07       Impact factor: 3.490

6.  Thiosulphate metabolism and rhodanese in Chromatium sp. strain D.

Authors:  A J Smith; J Lascelles
Journal:  J Gen Microbiol       Date:  1966-03
  6 in total

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