Literature DB >> 16349556

Laser microsurgery permits fungal plasma membrane single-ion-channel resolution at the hyphal tip.

A A Véry1, J M Davies.   

Abstract

A method for formation of high-electrical-resistance seals on the Neurospora crassa plasma membrane, allowing resolution of single-ion-channel activity by patch clamp electrophysiology, is reported. Laser microsurgery permits access to the hyphal apex without enzymatic cell wall digestion and loss of morphological polarity. Cell wall reformation is delayed by brefeldin. This method can allow full characterization of apical plasma membrane channels, which are implicated in tip growth.

Entities:  

Year:  1998        PMID： 16349556      PMCID： PMC106192     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell : Laser Microsurgery of the Fucus spiralis Rhizoid Cell Wall.

Authors:  A R Taylor; C Brownlee
Journal:  Plant Physiol       Date:  1992-08       Impact factor: 8.340

Review 2.  Multiple targets for brefeldin A.

Authors:  H R Pelham
Journal:  Cell       Date:  1991-11-01       Impact factor: 41.582

3.  Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro.

Authors:  Y Meyer; W Herth
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

4.  Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

Authors:  O P Hamill; A Marty; E Neher; B Sakmann; F J Sigworth
Journal:  Pflugers Arch       Date:  1981-08       Impact factor: 3.657

5.  A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.

Authors:  X L Zhou; M A Stumpf; H C Hoch; C Kung
Journal:  Science       Date:  1991-09-20       Impact factor: 47.728

6.  Effect of nonylphenol on growth of Neurospora crassa and Candida albicans.

Authors:  A J Karley; S I Powell; J M Davies
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

7.  Ion channel activity and tip growth: tip-localized stretch-activated channels generate an essential Ca2+ gradient in the oomycete Saprolegnia ferax.

Authors:  A Garrill; S L Jackson; R R Lew; I B Heath
Journal:  Eur J Cell Biol       Date:  1993-04       Impact factor: 4.492

8.  The effects of ruthenium red, lanthanum, fluorescein isothiocyanate and trifluoperazine on vesicle transport, vesicle fusion and tip extension in pollen tubes.

Authors:  J M Picton; M W Steer
Journal:  Planta       Date:  1985-01       Impact factor: 4.116

9.  Cytoskeletal regulation of ion channel distribution in the tip-growing organism Saprolegnia ferax.

Authors:  N N Levina; R R Lew; I B Heath
Journal:  J Cell Sci       Date:  1994-01       Impact factor: 5.285

10.  The roles of Ca2+ and plasma membrane ion channels in hyphal tip growth of Neurospora crassa.

Authors:  N N Levina; R R Lew; G J Hyde; I B Heath
Journal:  J Cell Sci       Date:  1995-11       Impact factor: 5.285
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  3 in total

1.  Outwardly rectifying anionic channel from the plasma membrane of the fungus Phycomyces blakesleeanus.

Authors:  Miroslav Zivić; Marko Popović; Natasa Todorović; Zeljko Vucinić
Journal:  Eukaryot Cell       Date:  2009-07-10

2.  Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs.

Authors:  A A Véry; J M Davies
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-15       Impact factor: 11.205

3.  TOK homologue in Neurospora crassa: first cloning and functional characterization of an ion channel in a filamentous fungus.

Authors:  Stephen K Roberts
Journal:  Eukaryot Cell       Date:  2003-02
  3 in total

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