Literature DB >> 16349472

Purification and Characterization of Two Epoxide Hydrolases from Corynebacterium sp. Strain N-1074.

T Nakamura1, T Nagasawa, F Yu, I Watanabe, H Yamada.   

Abstract

Enzymes II(a) and II(b), which catalyze the conversion of epichlorohydrin (ECH) to 3-chloro-1,2-propanediol (MCP), were purified from Corynebacterium sp. strain N-1074, which catalyzes the formation of (R)-MCP from prochiral 1,3-dichloro-2-propanol via ECH. The specific activity of enzyme II(a) for the formation of MCP from ECH was about 6.4-fold higher than that of enzyme II(b). Both enzymes catalyzed the conversion of 1,2-epoxides to the corresponding diol, although they differed in several enzymatic properties.

Entities:  

Year:  1994        PMID： 16349472      PMCID： PMC202036          DOI： 10.1128/aem.60.12.4630-4633.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Production of (R)-3-Chloro-1,2-Propanediol from Prochiral 1,3-Dichloro-2-Propanol by Corynebacterium sp. Strain N-1074.

Authors:  T Nakamura; F Yu; W Mizunashi; I Watanabe
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Characterization of the epoxide hydrolase from an epichlorohydrin-degrading Pseudomonas sp.

Authors:  M H Jacobs; A J Van den Wijngaard; M Pentenga; D B Janssen
Journal:  Eur J Biochem       Date:  1991-12-18

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Tartaric acid metabolism. IX. Synthesis with tartrate epoxidase.

Authors:  R H Allen; W B Jakoby
Journal:  J Biol Chem       Date:  1969-04-25       Impact factor: 5.157

6.  Cloning of two halohydrin hydrogen-halide-lyase genes of Corynebacterium sp. strain N-1074 and structural comparison of the genes and gene products.

Authors:  F Yu; T Nakamura; W Mizunashi; I Watanabe
Journal:  Biosci Biotechnol Biochem       Date:  1994-08       Impact factor: 2.043

Review 7.  Microsomal epoxide hydrolase. Properties, regulation and function.

Authors:  J Seidegård; J W DePierre
Journal:  Biochim Biophys Acta       Date:  1983-12-29

8.  Hydration of 9,10-epoxypalmitic acid by a soluble enzyme from Bacillus megaterium.

Authors:  B C Michaels; R T Ruettinger; A J Fulco
Journal:  Biochem Biophys Res Commun       Date:  1980-02-27       Impact factor: 3.575

9.  Characterization of a novel enantioselective halohydrin hydrogen-halide-lyase.

Authors:  T Nakamura; T Nagasawa; F Yu; I Watanabe; H Yamada
Journal:  Appl Environ Microbiol       Date:  1994-04       Impact factor: 4.792

10.  Resolution and some properties of enzymes involved in enantioselective transformation of 1,3-dichloro-2-propanol to (R)-3-chloro-1,2-propanediol by Corynebacterium sp. strain N-1074.

Authors:  T Nakamura; T Nagasawa; F Yu; I Watanabe; H Yamada
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490
  10 in total
  2 in total

1.  Metabolism of 2-methylpropene (isobutylene) by the aerobic bacterium Mycobacterium sp. strain ELW1.

Authors:  Samanthi Kottegoda; Elizabeth Waligora; Michael Hyman
Journal:  Appl Environ Microbiol       Date:  2015-01-09       Impact factor: 4.792

2.  Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14 belongs to a novel class of epoxide hydrolases.

Authors:  M J van der Werf; K M Overkamp; J A de Bont
Journal:  J Bacteriol       Date:  1998-10       Impact factor: 3.490
  2 in total

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