Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633.

Literature DB >> 16349130

Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633.

H F Hermes¹, T Sonke, P J Peters, J A van Balken, J Kamphuis, L Dijkhuizen, E M Meijer.

Abstract

An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and alpha-H amino acid amides, e.g., l-phenylglycine amide.

Entities: Chemical Species

Year: 1993 PMID： 16349130 PMCID： PMC195905 DOI： 10.1128/aem.59.12.4330-4334.1993

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

8 in total

1. The thiobacilli.

Authors: W VISHNIAC; M SANTER
Journal: Bacteriol Rev Date: 1957-09

Review 2. New developments in the chemo-enzymatic production of amino acids.

Authors: J Kamphuis; W H Boesten; Q B Broxterman; H F Hermes; J A van Balken; E M Meijer; H E Schoemaker
Journal: Adv Biochem Eng Biotechnol Date: 1990 Impact factor: 2.635

3. Isolation and characterization of an intracellular aminopeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.

Authors: M Hanner; B Redl; G Stöffler
Journal: Biochim Biophys Acta Date: 1990-02-26

Review 4. Applications of stereoselectivity of enzymes: synthesis of optically active amino acids and alpha-hydroxy acids, and stereospecific isotope-labeling of amino acids, amines and coenzymes.

Authors: K Yonaha; K Soda
Journal: Adv Biochem Eng Biotechnol Date: 1986 Impact factor: 2.635

Review 5. Peptidases and proteases of Escherichia coli and Salmonella typhimurium.

Authors: A M Lazdunski
Journal: FEMS Microbiol Rev Date: 1989-09 Impact factor: 16.408

Review 6. Microbial proteinases.

Authors: H M Kalisz
Journal: Adv Biochem Eng Biotechnol Date: 1988 Impact factor: 2.635

Review 7. Comparative specificity of microbial proteinases.

Authors: K Morihara
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1974

8. Determination of the enantiomers of alpha-amino acids and alpha-amino acid amides by high-performance liquid chromatography with a chiral mobile phase.

Authors: A Duchateau; M Crombach; M Aussems; J Bongers
Journal: J Chromatogr Date: 1989-01-06

8 in total

9 in total

1. Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.

Authors: S C Woolwine; D J Wozniak
Journal: J Bacteriol Date: 1999-01 Impact factor: 3.490

Purification and Characterization of an l-Aminopeptidase from Pseudomonas putida ATCC 12633.

1. The thiobacilli.

Review 2. New developments in the chemo-enzymatic production of amino acids.

3. Isolation and characterization of an intracellular aminopeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.

Review 4. Applications of stereoselectivity of enzymes: synthesis of optically active amino acids and alpha-hydroxy acids, and stereospecific isotope-labeling of amino acids, amines and coenzymes.

Review 5. Peptidases and proteases of Escherichia coli and Salmonella typhimurium.

Review 6. Microbial proteinases.

Review 7. Comparative specificity of microbial proteinases.

8. Determination of the enantiomers of alpha-amino acids and alpha-amino acid amides by high-performance liquid chromatography with a chiral mobile phase.

1. Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.

Review 2. Natural neo acids and neo alkanes: their analogs and derivatives.

3. L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321.

4. Purification and characterization of hyperthermotolerant leucine aminopeptidase from Geobacillus thermoleovorans 47b.

5. Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire.

6. A novel amidase (half-amidase) for half-amide hydrolysis involved in the bacterial metabolism of cyclic imides.

7. Regulation of the activity of intracellular alanylaminopeptidase synthesized by Pseudomonas sp.

8. Characterization of an α-amino-ɛ-caprolactam racemase with broad substrate specificity from Citreicella sp. SE45.

Review 9. Industrial biotechnology of Pseudomonas putida: advances and prospects.