Literature DB >> 16349054

Purification and Properties of a Highly Active Organophosphorus Acid Anhydrolase from Alteromonas undina.

T C Cheng1, S P Harvey, A N Stroup.   

Abstract

A highly active organophosphorus acid anhydrolase from Alteromonas undina was purified to homogeneity and found to be composed of a single polypeptide chain with a molecular weight of 53,000. With diisopropylfluorophosphate as a substrate, the purified enzyme has a specific activity of approximately 575 mumol/min/mg of protein. The enzyme has optimum activity at pH 8.0 and 55 degrees C and is stimulated by sulfhydryl reducing agents and manganese. It is capable of rapidly hydrolyzing a wide range of nerve agents and several chromogenic phosphinates.

Entities:  

Year:  1993        PMID： 16349054      PMCID： PMC182420          DOI： 10.1128/aem.59.9.3138-3140.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate.

Authors:  J J DeFrank; T C Cheng
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

2.  Organofluorophosphate-hydrolyzing activity in an estuarine clam, Rangia cuneata.

Authors:  R S Anderson; H D Durst; W G Landis
Journal:  Comp Biochem Physiol C Comp Pharmacol Toxicol       Date:  1988

3.  Bacterial detoxification of diisopropyl fluorophosphate.

Authors:  H Attaway; J O Nelson; A M Baya; M J Voll; W E White; D J Grimes; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Inhibition of a soman- and diisopropyl phosphorofluoridate (DFP)-detoxifying enzyme by Mipafox.

Authors:  F C Hoskin
Journal:  Biochem Pharmacol       Date:  1985-06-15       Impact factor: 5.858

6.  Discovery of multiple organofluorophosphate hydrolyzing activities in the protozoan Tetrahymena thermophila.

Authors:  W G Landis; D M Haley; M V Haley; D W Johnson; H D Durst; R E Savage
Journal:  J Appl Toxicol       Date:  1987-02       Impact factor: 3.446

7.  Hydrolysis of nerve gas by squid-type diisopropyl phosphorofluoridate hydrolyzing enzyme on agarose resin.

Authors:  F C Hoskin; A H Roush
Journal:  Science       Date:  1982-03-05       Impact factor: 47.728
  7 in total
  15 in total

1.  Nano-intercalated organophosphorus-hydrolyzing enzymes in organophosphorus antagonism.

Authors:  Ilona Petrikovics; Melinda Wales; Marianna Budai; Jorn C C Yu; Mária Szilasi
Journal:  AAPS PharmSciTech       Date:  2011-12-09       Impact factor: 3.246

2.  A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties.

Authors:  Luigia Merone; Luigi Mandrich; Mosè Rossi; Giuseppe Manco
Journal:  Extremophiles       Date:  2005-05-21       Impact factor: 2.395

3.  Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  M Ghosh; A M Grunden; D M Dunn; R Weiss; M W Adams
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

Review 4.  Organophosphate-Hydrolyzing Enzymes as First-Line of Defence Against Nerve Agent-Poisoning: Perspectives and the Road Ahead.

Authors:  A R Satvik Iyengar; Abhay H Pande
Journal:  Protein J       Date:  2016-12       Impact factor: 2.371

Review 5.  Organophosphorus-degrading bacteria: ecology and industrial applications.

Authors:  Brajesh K Singh
Journal:  Nat Rev Microbiol       Date:  2008-12-22       Impact factor: 60.633

6.  Identification of an opd (organophosphate degradation) gene in an Agrobacterium isolate.

Authors:  Irene Horne; Tara D Sutherland; Rebecca L Harcourt; Robyn J Russell; John G Oakeshott
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

7.  Crystallization and preliminary X-ray diffraction analysis of the hyperthermophilic Sulfolobus solfataricus phosphotriesterase.

Authors:  Mikael Elias; Jérôme Dupuy; Luigia Merone; Claude Lecomte; Mosè Rossi; Patrick Masson; Giuseppe Manco; Eric Chabriere
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-06-11

8.  Crystallization and preliminary X-ray diffraction analysis of Xaa-Pro dipeptidase from Xanthomonas campestris.

Authors:  Ashwani Kumar; Venkata Narayana Are; Biplab Ghosh; Utsavi Agrawal; Sahayog N Jamdar; Ravindra D Makde; Surinder M Sharma
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-08-27       Impact factor: 1.056

9.  Cloning and expression of a gene encoding a bacterial enzyme for decontamination of organophosphorus nerve agents and nucleotide sequence of the enzyme.

Authors:  T C Cheng; S P Harvey; G L Chen
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792

10.  Structural determinants of the high thermal stability of SsoPox from the hyperthermophilic archaeon Sulfolobus solfataricus.

Authors:  Pompea Del Vecchio; Mikael Elias; Luigia Merone; Giuseppe Graziano; Jérôme Dupuy; Luigi Mandrich; Paola Carullo; Bertrand Fournier; Daniel Rochu; Mosè Rossi; Patrick Masson; Eric Chabriere; Giuseppe Manco
Journal:  Extremophiles       Date:  2009-02-27       Impact factor: 2.395
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