| Literature DB >> 16349024 |
G K Podila1, S Kotagiri, S Shantharam.
Abstract
A heterologous gene probe encoding the alpha and beta subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.Entities:
Year: 1993 PMID: 16349024 PMCID: PMC182344 DOI: 10.1128/aem.59.8.2717-2719.1993
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792