Literature DB >> 16348594

Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus.

N Touhara1, H Taguchi, Y Koyama, T Ohta, H Matsuzawa.   

Abstract

Aqualysin I is synthesized as a large precursor, processed, and secreted into the culture medium by Thermus aquaticus YT-1. An expression plasmid for the aqualysin I gene in T. thermophilus HB27 was constructed. T. thermophilus cells harboring the recombinant plasmid produced correctly processed aqualysin I, and the mature enzyme was secreted into the culture medium.

Entities:  

Year:  1991        PMID： 16348594      PMCID： PMC183977          DOI： 10.1128/aem.57.11.3385-3387.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.

Authors:  J Norrander; T Kempe; J Messing
Journal:  Gene       Date:  1983-12       Impact factor: 3.688

3.  Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease.

Authors:  J Pohlner; R Halter; K Beyreuther; T F Meyer
Journal:  Nature       Date:  1987 Jan 29-Feb 4       Impact factor: 49.962

4.  Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal pro-sequences and its processing in Escherichia coli.

Authors:  I Terada; S T Kwon; Y Miyata; H Matsuzawa; T Ohta
Journal:  J Biol Chem       Date:  1990-04-25       Impact factor: 5.157

5.  Extracellular transport of cholera toxin B subunit using Neisseria IgA protease beta-domain: conformation-dependent outer membrane translocation.

Authors:  T Klauser; J Pohlner; T F Meyer
Journal:  EMBO J       Date:  1990-06       Impact factor: 11.598

6.  Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1.

Authors:  S T Kwon; H Matsuzawa; T Ohta
Journal:  J Biochem       Date:  1988-10       Impact factor: 3.387

7.  Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1.

Authors:  H Matsuzawa; K Tokugawa; M Hamaoki; M Mizoguchi; H Taguchi; I Terada; S T Kwon; T Ohta
Journal:  Eur J Biochem       Date:  1988-02-01

8.  Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

Authors:  Y Koyama; T Hoshino; N Tomizuka; K Furukawa
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

9.  Characterization of the precursor of Serratia marcescens serine protease and COOH-terminal processing of the precursor during its excretion through the outer membrane of Escherichia coli.

Authors:  H Miyazaki; N Yanagida; S Horinouchi; T Beppu
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

10.  Isolation of extrachromosomal deoxyribonucleic acids from extremely thermophilic bacteria.

Authors:  F Hishinuma; T Tanaka; K Sakaguchi
Journal:  J Gen Microbiol       Date:  1978-02
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  3 in total

1.  Production of recombinant alpha-galactosidases in Thermus thermophilus.

Authors:  O Fridjonsson; R Mattes
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

2.  A new Thermus-Escherichia coli shuttle integration vector system.

Authors:  M Tamakoshi; M Uchida; K Tanabe; S Fukuyama; A Yamagishi; T Oshima
Journal:  J Bacteriol       Date:  1997-08       Impact factor: 3.490

3.  Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus.

Authors:  Marta Irla; Eivind B Drejer; Trygve Brautaset; Sigrid Hakvåg
Journal:  Microb Cell Fact       Date:  2020-07-28       Impact factor: 5.328
  3 in total

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