Literature DB >> 16348453

Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography.

J Siebert1, R J Palmer, P Hirsch.   

Abstract

A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.

Entities:  

Year:  1991        PMID： 16348453      PMCID： PMC182811          DOI： 10.1128/aem.57.3.879-881.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Biomass of the cryptoendolithic microbiota from the Antarctic desert.

Authors:  J R Vestal
Journal:  Appl Environ Microbiol       Date:  1988-04       Impact factor: 4.792

2.  The cryptoendolithic microbial environment in the Ross Desert of Antarctica: satellite-transmitted continuous nanoclimate data, 1984 to 1986.

Authors:  E I Friedmann; C P McKay; J A Nienow
Journal:  Polar Biol       Date:  1987       Impact factor: 2.310

3.  Endolithic microorganisms in the antarctic cold desert.

Authors:  E I Friedmann
Journal:  Science       Date:  1982-02-26       Impact factor: 47.728

4.  Diel production and microheterotrophic utilization of dissolved free amino acids in waters off southern california.

Authors:  A F Carlucci; D B Craven; S M Henrichs
Journal:  Appl Environ Microbiol       Date:  1984-07       Impact factor: 4.792

5.  Specific uptake rates of amino acids by attached and free-living bacteria in a mesotrophic lake.

Authors:  M Simon
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

6.  A theoretical study on the amount of ATP required for synthesis of microbial cell material.

Authors:  A H Stouthamer
Journal:  Antonie Van Leeuwenhoek       Date:  1973       Impact factor: 2.271

7.  Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.

Authors:  R L Heinrikson; S C Meredith
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

8.  Inorganic and organic phosphate measurements in the nanomolar range.

Authors:  P P Van Veldhoven; G P Mannaerts
Journal:  Anal Biochem       Date:  1987-02-15       Impact factor: 3.365
  8 in total
  4 in total

1.  Long-term productivity in the cryptoendolithic microbial community of the Ross Desert, Antarctica.

Authors:  E I Friedmann; L Kappen; M A Meyer; J A Nienow
Journal:  Microb Ecol       Date:  1993 Jan-Feb       Impact factor: 4.552

2.  Peter Hirsch and biofilms: microbial ecology's role in a "new" field.

Authors:  R J Palmer
Journal:  Microb Ecol       Date:  2003-10-31       Impact factor: 4.552

Review 3.  Cyanobacteria and biodeterioration of cultural heritage: a review.

Authors:  C A Crispim; C C Gaylarde
Journal:  Microb Ecol       Date:  2004-09-23       Impact factor: 4.552

4.  Biomass and organic acids in sandstone of a weathering building: Production by bacterial and fungal isolates.

Authors:  R J Palmer; J Siebert; P Hirsch
Journal:  Microb Ecol       Date:  1991-12       Impact factor: 4.552
  4 in total

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