Literature DB >> 16348267

Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase.

Shinsaku Hayashida1, Yuji Teramoto, Takehiro Inoue, Shinji Mitsuiki.   

Abstract

alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase.

Entities:  

Year:  1990        PMID： 16348267      PMCID： PMC184772          DOI： 10.1128/aem.56.8.2584-2586.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  5 in total

1.  Production and Characteristics of Raw-Starch-Digesting alpha-Amylase from a Protease-Negative Aspergillus ficum Mutant.

Authors:  S Hayashida; Y Teramoto
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

2.  Production and Characteristics of Raw-Potato-Starch-Digesting alpha-Amylase from Bacillus subtilis 65.

Authors:  S Hayashida; Y Teramoto; T Inoue
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

3.  Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis.

Authors:  P Tomme; H Van Tilbeurgh; G Pettersson; J Van Damme; J Vandekerckhove; J Knowles; T Teeri; M Claeyssens
Journal:  Eur J Biochem       Date:  1988-01-04

4.  Immobilization of ligands for biospecific affinity chromatography via their hydroxyl groups. The cyclohexaamylose-beta-amylase system.

Authors:  P Vretblad
Journal:  FEBS Lett       Date:  1974-10-01       Impact factor: 4.124

5.  Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II.

Authors:  T T Teeri; P Lehtovaara; S Kauppinen; I Salovuori; J Knowles
Journal:  Gene       Date:  1987       Impact factor: 3.688
  5 in total
  5 in total

1.  Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli.

Authors:  Chii-Ling Jeang; Li-Shien Chen; Ming-Yu Chen; Rong-Jen Shiau
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

2.  Raw-starch-digesting and thermostable alpha-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing.

Authors:  H Iefuji; M Chino; M Kato; Y Iimura
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

3.  Isolation of a raw starch-binding fragment from barley alpha-amylase.

Authors:  D W Wong; S B Batt; B K Tibbot; G H Robertson
Journal:  J Protein Chem       Date:  2000-07

4.  Purification and characterization of a truncated Bacillus subtilis alpha-amylase produced by Escherichia coli.

Authors:  J L Marco; L A Bataus; F F Valência; C J Ulhoa; S Astolfi-Filho; C R Felix
Journal:  Appl Microbiol Biotechnol       Date:  1996-02       Impact factor: 4.813

5.  Analysis of the raw starch-binding domain by mutation of a glucoamylase from Aspergillus awamori var. kawachi expressed in Saccharomyces cerevisiae.

Authors:  M Goto; T Semimaru; K Furukawa; S Hayashida
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792
  5 in total

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