Literature DB >> 16348077

Cloning and Expression of a Schwanniomyces occidentalis alpha-Amylase Gene in Saccharomyces cerevisiae.

T T Wang1, L L Lin, W H Hsu.   

Abstract

An alpha-amylase gene (AMY) was cloned from Schwanniomyces occidentalis CCRC 21164 into Saccharomyces cerevisiae AH22 by inserting Sau3AI-generated DNA fragments into the BamHI site of YEp16. The 5-kilobase insert was shown to direct the synthesis of alpha-amylase. After subclones containing various lengths of restricted fragments were screened, a 3.4-kilobase fragment of the donor strain DNA was found to be sufficient for alpha-amylase synthesis. The concentration of alpha-amylase in culture broth produced by the S. cerevisiae transformants was about 1.5 times higher than that of the gene donor strain. The secreted alpha-amylase was shown to be indistinguishable from that of Schwanniomyces occidentalis on the basis of molecular weight and enzyme properties.

Entities:  

Year:  1989        PMID： 16348077      PMCID： PMC203241          DOI： 10.1128/aem.55.12.3167-3172.1989

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

Review 1.  The 2 micron circle plasmid of Saccharomyces cerevisiae.

Authors:  A B Futcher
Journal:  Yeast       Date:  1988-03       Impact factor: 3.239

2.  alpha-Amylase gene of Streptomyces limosus: nucleotide sequence, expression motifs, and amino acid sequence homology to mammalian and invertebrate alpha-amylases.

Authors:  C M Long; M J Virolle; S Y Chang; S Chang; M J Bibb
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.

Authors:  S A Lacks; S S Springhorn
Journal:  J Biol Chem       Date:  1980-08-10       Impact factor: 5.157

5.  Expression of alpha-amylase in Bacillus licheniformis.

Authors:  D M Rothstein; P E Devlin; R L Cate
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

6.  The mouse alpha-amylase multigene family. Sequence organization of members expressed in the pancreas, salivary gland and liver.

Authors:  U Schibler; A C Pittet; R A Young; O Hagenbüchle; M Tosi; S Gellman; P K Wellauer
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

7.  Cloning, characterization and regulation of an alpha-amylase gene from Streptomyces limosus.

Authors:  M J Virolle; M J Bibb
Journal:  Mol Microbiol       Date:  1988-03       Impact factor: 3.501

8.  Transformation of yeast.

Authors:  A Hinnen; J B Hicks; G R Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

9.  Efficient synthesis and secretion of a thermophilic alpha-amylase by protein-producing Bacillus brevis 47 carrying the Bacillus stearothermophilus amylase gene.

Authors:  N Tsukagoshi; S Iritani; T Sasaki; T Takemura; H Ihara; Y Idota; H Yamagata; S Udaka
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

10.  A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1 beta in Saccharomyces cerevisiae.

Authors:  C Baldari; J A Murray; P Ghiara; G Cesareni; C L Galeotti
Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598
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  4 in total

1.  Raw-starch-digesting and thermostable alpha-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing.

Authors:  H Iefuji; M Chino; M Kato; Y Iimura
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

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Journal:  World J Microbiol Biotechnol       Date:  2015-07-12       Impact factor: 3.312

3.  The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus.

Authors:  Shenglin Hu; Zhefan Wang; Dongmei Wang; Jichao Wang; Jiong Hong
Journal:  3 Biotech       Date:  2021-08-18       Impact factor: 2.893

Review 4.  Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).

Authors:  Justyna Ruchala; Olena O Kurylenko; Kostyantyn V Dmytruk; Andriy A Sibirny
Journal:  J Ind Microbiol Biotechnol       Date:  2019-10-21       Impact factor: 3.346
  4 in total

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