Literature DB >> 16347044

Cloning and Expression in Escherichia coli of the Polysaccharide Depolymerase Associated with Bacteriophage-Infected Erwinia amylovora.

P A Vandenbergh1, R L Cole.   

Abstract

The bacteriophage-encoded polysaccharide depolymerase produced in Erwinia amylovora has been cloned and expressed in Escherichia coli. The bacteriophage ERA103 genome was observed to consist of five EcoRI fragments, labeled as follows: A, 7.5 kilobases (kb); B, 5.0 kb; C, 2.7 kb; D, 2.1 kb; and E, 1.8 kb. A restriction map for ERA103 was also prepared. Each of the fragments were cloned into the positive-selection vector pOP203(A(2)) and pBR322.

Entities:  

Year:  1986        PMID： 16347044      PMCID： PMC238974          DOI： 10.1128/aem.51.4.862-864.1986

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis.

Authors:  P V LIU; Y ABE; J L BATES
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Authors:  R A FAIRBRIDGE; K J WILLIS; R G BOOTH
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3.  Partial Purification and Characterization of a Polysaccharide Depolymerase Associated with Phage-Infected Erwinia amylovora.

Authors:  P A Vandenbergh; A M Wright; A K Vidaver
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Journal:  Appl Environ Microbiol       Date:  1979-10       Impact factor: 4.792

5.  Iron-chelating compounds produced by soil pseudomonads: correlation with fungal growth inhibition.

Authors:  P A Vandenbergh; C F Gonzalez; A M Wright; B S Kunka
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6.  Characteristics of Erwinia amylovora bacteriophage and its possible role in the epidemology of fire blight.

Authors:  J M Erskine
Journal:  Can J Microbiol       Date:  1973-07       Impact factor: 2.419

7.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

Authors:  K R Yamamoto; B M Alberts; R Benzinger; L Lawhorne; G Treiber
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

8.  Sensitive biuret method for determination of protein in an impure system such as whole bacteria.

Authors:  A L Koch; S L Putnam
Journal:  Anal Biochem       Date:  1971-11       Impact factor: 3.365

9.  Overproduction of bacteriophage Q beta maturation (A2) protein leads to cell lysis.

Authors:  R B Winter; L Gold
Journal:  Cell       Date:  1983-07       Impact factor: 41.582

10.  Plasmid content of some oral microorganisms isolated from subgingival plaque.

Authors:  P A Vandenbergh; S A Syed; C F Gonzalez; W J Loesche; R H Olsen
Journal:  J Dent Res       Date:  1982-03       Impact factor: 6.116
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  3 in total

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2.  Bacteriophages LIMElight and LIMEzero of Pantoea agglomerans, belonging to the "phiKMV-like viruses".

Authors:  Evelien M Adriaenssens; Pieter-Jan Ceyssens; Vincent Dunon; Hans-Wolfgang Ackermann; Johan Van Vaerenbergh; Martine Maes; Maurice De Proft; Rob Lavigne
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3.  Molecular and physiological properties of bacteriophages from North America and Germany affecting the fire blight pathogen Erwinia amylovora.

Authors:  Ina Müller; Rudi Lurz; Michael Kube; Claudia Quedenau; Wilhelm Jelkmann; Klaus Geider
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  3 in total

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