Literature DB >> 16346595

Method for Growth and Purification of Bacteriophage 643 on Streptococcus lactis ML3.

D J Lyttle1, G B Petersen.   

Abstract

The yield of bacteriophage 643 was increased by infecting cultures of Streptococcus lactis ML3 in late-log phase growth, harvesting the infected cells, and suspending them in fresh, phosphate-buffered minimal medium. The cells lysed after this treatment and produced high titers of bacteriophage. The phage particles were dissociated from debris by 2 M NaCl and purified by differential and CsCl band centrifugation.

Entities:  

Year:  1984        PMID： 16346595      PMCID： PMC240388          DOI： 10.1128/aem.48.1.242-244.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  5 in total

1.  Band-centrifugation of macromolecules and viruses in self-generating density gradients.

Authors:  J VINOGRAD; R BRUNER; R KENT; J WEIGLE
Journal:  Proc Natl Acad Sci U S A       Date:  1963-06       Impact factor: 11.205

2.  Genetic variation in the sex factor of Escherichia coli.

Authors:  E A ADELBERG; S N BURNS
Journal:  J Bacteriol       Date:  1960-03       Impact factor: 3.490

3.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

4.  Preparation and storage of high-titer lactic streptococcus bacteriophages.

Authors:  J Nyiendo; R J Seidler; W E Sandine; P R Elliker
Journal:  Appl Microbiol       Date:  1974-01

5.  Improved medium for lactic streptococci and their bacteriophages.

Authors:  B E Terzaghi; W E Sandine
Journal:  Appl Microbiol       Date:  1975-06
  5 in total

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