Adsorption of Bacteriophage eb7 on Streptococcus cremoris EB7.

When M17 broth was used as growth medium before preparation of cell walls, adsorption of phage eb7 on Streptococcus cremoris EB7 at pH 4.0 was stimulated. When preparation included treatment with trypsin, absorption of phage was 65%. Without trypsin treatment, absorption was 81%. Only 10 to 20% of the adsorption was irreversible. Treatment with pepsin or commercial rennet but not pure chymosin prevented adsorption on non-trypsin-treated cell walls. d-Galactosamine treatment of phage eb7 had an inactivating effect which was enhanced by l-rhamnose.