Literature DB >> 16345999

Tetrazolium reduction-malachite green method for assessing the viability of filamentous bacteria in activated sludge.

G Bitton1, B Koopman.   

Abstract

A method was developed to assess the activity of filamentous bacteria in activated sludge. It involves the incubation of activated sludge with 2(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride followed by staining with malachite green. Both cells and 2(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride-formazan crystals can be observed in prepared specimens by using bright-field microscopy. This procedure allowed us to distinguish between inactive and actively metabolizing filaments after chlorine application to control the bulking of activated sludge.

Entities:  

Year:  1982        PMID： 16345999      PMCID： PMC241950          DOI： 10.1128/aem.43.4.964-966.1982

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Enumeration of particle-bound and unattached respiring bacteria in the salt marsh environment.

Authors:  R W Harvey; L Y Young
Journal:  Appl Environ Microbiol       Date:  1980-07       Impact factor: 4.792

2.  Comparison of two direct-count methods for determining metabolizing bacteria in freshwater.

Authors:  J S Maki; C C Remsen
Journal:  Appl Environ Microbiol       Date:  1981-05       Impact factor: 4.792

3.  A tentative direct microscopic method for counting living marine bacteria.

Authors:  K Kogure; U Simidu; N Taga
Journal:  Can J Microbiol       Date:  1979-03       Impact factor: 2.419

4.  Use of nuclepore filters for counting bacteria by fluorescence microscopy.

Authors:  J E Hobbie; R J Daley; S Jasper
Journal:  Appl Environ Microbiol       Date:  1977-05       Impact factor: 4.792

5.  Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration.

Authors:  R Zimmermann; R Iturriaga; J Becker-Birck
Journal:  Appl Environ Microbiol       Date:  1978-12       Impact factor: 4.792

6.  Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural water.

Authors:  L A Meyer-Reil
Journal:  Appl Environ Microbiol       Date:  1978-09       Impact factor: 4.792
  6 in total
  8 in total

1.  Structure and dynamics of microbial community in full-scale activated sludge reactors.

Authors:  Taewoo Yi; Eun-Hee Lee; Shinyoung Kang; Jikyung Shin; Kyung-Suk Cho
Journal:  J Ind Microbiol Biotechnol       Date:  2011-06-02       Impact factor: 3.346

2.  Detection of Active Yeast Cells (Saccharomyces cerevisiae) in Frozen Dough Sections.

Authors:  K Autio; T Mattila-Sandholm
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

3.  A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater.

Authors:  L K King; B C Parker
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

4.  Comparison of methods for detection and enumeration of airborne microorganisms collected by liquid impingement.

Authors:  S Terzieva; J Donnelly; V Ulevicius; S A Grinshpun; K Willeke; G N Stelma; K P Brenner
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

5.  Baker's yeast assay procedure for testing heavy metal toxicity.

Authors:  G Bitton; B Koopman; H D Wang
Journal:  Bull Environ Contam Toxicol       Date:  1984-01       Impact factor: 2.151

6.  Chlorine-susceptible and chlorine-resistant type 021N bacteria occurring in bulking activated sludges.

Authors:  M A Séka; Y Kalogo; F Hammes; J Kielemoes; W Verstraete
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

7.  Malachite green-INT (MINT) method for determining active bacteria in sewage.

Authors:  R J Dutton; G Bitton; B Koopman
Journal:  Appl Environ Microbiol       Date:  1983-12       Impact factor: 4.792

8.  Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water.

Authors:  G Schaule; H C Flemming; H F Ridgway
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792
  8 in total

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