Literature DB >> 16345955

Simplified microassay for pullulan synthesis.

M A Finkelman1, A Vardanis.   

Abstract

A simple radiometric microassay for extracellular polysaccharide elaboration by yeast-like cells of Aureobasidium pullulans was developed, based upon a procedure originally described by Catley (FEBS Lett. 20:174-176, 1972). Incorporation of [C]glucose into pullulan was linear with respect to time and cell dose. The pH and temperature optima for elaboration were 5.3 and 30 degrees C, respectively. Polysaccharide elaboration declined linearly with culture age.

Entities:  

Year:  1982        PMID： 16345955      PMCID： PMC241852          DOI： 10.1128/aem.43.2.483-485.1982

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Pullulan elaboration, an inducible system of Pullularia pullulans.

Authors:  B J. Catley
Journal:  FEBS Lett       Date:  1972-02-01       Impact factor: 4.124

2.  Pullulan, a relationship between molecular weight and fine structure.

Authors:  B J. Catley
Journal:  FEBS Lett       Date:  1970-10-05       Impact factor: 4.124

3.  The rate of elaboration of the extracellular polysaccharide, pullulan, during growth of Pullularia pullulans.

Authors:  B J Catley
Journal:  J Gen Microbiol       Date:  1973-09

4.  Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans.

Authors:  B J Catley
Journal:  Appl Microbiol       Date:  1971-10

5.  A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucose.

Authors:  J A Thomas; K K Schlender; J Larner
Journal:  Anal Biochem       Date:  1968-10-24       Impact factor: 3.365

6.  Flocculant and chemical properties of a polysaccharide from Pullularia pullulans.

Authors:  J E Zajic; A LeDuy
Journal:  Appl Microbiol       Date:  1973-04

7.  Utilization of carbon sources by Pullularia pullulans for the elaboration of extracellular polysaccharides.

Authors:  B J Catley
Journal:  Appl Microbiol       Date:  1971-10
  7 in total
  2 in total

1.  Pullulan Elaboration by Aureobasidium pullulans Protoplasts.

Authors:  M A Finkelman; A Vardanis
Journal:  Appl Environ Microbiol       Date:  1982-07       Impact factor: 4.792

2.  Biosynthesis and hyper production of pullulan by a newly isolated strain of Aspergillus japonicus-VIT-SB1.

Authors:  Bishwambhar Mishra; V Suneetha
Journal:  World J Microbiol Biotechnol       Date:  2014-03-08       Impact factor: 3.312
  2 in total

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