Literature DB >> 16345570

Acetylene reduction assay for nitrogenase activity: gas chromatographic determination of ethylene per sample in less than one minute.

K A David1, S K Apte, A Banerji, J Thomas.   

Abstract

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rate of about 13 samples in 10 min.

Entities:  

Year:  1980        PMID： 16345570      PMCID： PMC291480          DOI： 10.1128/aem.39.5.1078-1080.1980

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Effects of long-term treatment with acetylene on nitrogen-fixing microorganisms.

Authors:  K A David; P Fay
Journal:  Appl Environ Microbiol       Date:  1977-12       Impact factor: 4.792

2.  ATP-dependent reduction of azide and HCN by N2-fixing enzymes of Azotobacter vinelandii and Clostridium pasteurianum.

Authors:  R W Hardy; E Knight
Journal:  Biochim Biophys Acta       Date:  1967-05-16

3.  In situ studies on N2 fixation using the acetylene reduction technique.

Authors:  W D Stewart; G P Fitzgerald; R H Burris
Journal:  Proc Natl Acad Sci U S A       Date:  1967-11       Impact factor: 11.205

4.  Absence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga.

Authors:  J Thomas
Journal:  Nature       Date:  1970-10-10       Impact factor: 49.962

5.  Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum.

Authors:  M J Dilworth
Journal:  Biochim Biophys Acta       Date:  1966-10-31

6.  The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.

Authors:  R W Hardy; R D Holsten; E K Jackson; R C Burns
Journal:  Plant Physiol       Date:  1968-08       Impact factor: 8.340
  6 in total
  9 in total

1.  High radiation and desiccation tolerance of nitrogen-fixing cultures of the cyanobacterium Anabaena sp. strain PCC 7120 emanates from genome/proteome repair capabilities.

Authors:  Harinder Singh; Kirti Anurag; Shree Kumar Apte
Journal:  Photosynth Res       Date:  2013-10-12       Impact factor: 3.573

2.  Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria.

Authors:  S K Apte; B R Reddy; J Thomas
Journal:  Appl Environ Microbiol       Date:  1987-08       Impact factor: 4.792

3.  INCREASING NODULE SIZE1 Expression Is Required for Normal Rhizobial Symbiosis and Nodule Development.

Authors:  Xinxin Li; Jiakun Zheng; Yongqing Yang; Hong Liao
Journal:  Plant Physiol       Date:  2018-09-28       Impact factor: 8.340

4.  Possible implications of reciprocity between ethylene and aflatoxin biogenesis in Aspergillus flavus and Aspergillus parasiticus.

Authors:  A Sharma; S R Padwal-Desai; G B Nadkarni
Journal:  Appl Environ Microbiol       Date:  1985-01       Impact factor: 4.792

5.  Desert gerbils affect bacterial composition of soil.

Authors:  Tatyana A Kuznetsova; Michael Kam; Irina S Khokhlova; Natalia V Kostina; Tatiana G Dobrovolskaya; Marat M Umarov; A Allan Degen; Georgy I Shenbrot; Boris R Krasnov
Journal:  Microb Ecol       Date:  2013-07-16       Impact factor: 4.552

6.  Unusual radioresistance of nitrogen-fixing cultures of Anabaena strains.

Authors:  Harinder Singh; Tonina Fernandes; Shree Kumar Apte
Journal:  J Biosci       Date:  2010-09       Impact factor: 1.826

7.  Excess nitrate induces nodule greening and reduces transcript and protein expression levels of soybean leghaemoglobins.

Authors:  Mengke Du; Zhi Gao; Xinxin Li; Hong Liao
Journal:  Ann Bot       Date:  2020-06-19       Impact factor: 4.357

8.  In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial mats.

Authors:  Anne-Soisig Steunou; Devaki Bhaya; Mary M Bateson; Melanie C Melendrez; David M Ward; Eric Brecht; John W Peters; Michael Kühl; Arthur R Grossman
Journal:  Proc Natl Acad Sci U S A       Date:  2006-02-07       Impact factor: 11.205

9.  Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan.

Authors:  Sughra Hakim; Asma Imran; M Sajjad Mirza
Journal:  Braz J Microbiol       Date:  2020-11-03       Impact factor: 2.476
  9 in total

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