Literature DB >> 16345522

Density Centrifugation Method for Recovering Rhizobium spp. from Soil for Fluorescent-Antibody Studies.

A G Wollum1, R H Miller.   

Abstract

A density centrifugation procedure has been developed as a replacement for soil flocculation and clarification steps employed in quantitative fluorescent-antibody studies on Rhizobium in soils. Near-quantitative recovery of added cells of two strains of Rhizobium japonicum and two strains of R. phaseoli was achieved from six soils with various properties. It is proposed that this technique may prove useful in separating other soil microorganisms from soil particles in ecological studies employing fluorescent-antibody techniques.

Entities:  

Year:  1980        PMID： 16345522      PMCID： PMC291356          DOI： 10.1128/aem.39.2.466-469.1980

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Population Densities of Rhizobium japonicum Strain 123 Estimated Directly in Soil and Rhizospheres.

Authors:  V G Reyes; E L Schmidt
Journal:  Appl Environ Microbiol       Date:  1979-05       Impact factor: 4.792

2.  Use of nuclepore filters for counting bacteria by fluorescence microscopy.

Authors:  J E Hobbie; R J Daley; S Jasper
Journal:  Appl Environ Microbiol       Date:  1977-05       Impact factor: 4.792

3.  Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum.

Authors:  M A Cole; G H Elkan
Journal:  Antimicrob Agents Chemother       Date:  1973-09       Impact factor: 5.191

4.  Autoradiography and immunofluorescence combined for autecological study of single cell activity with Nitrobacter as a model system.

Authors:  C B Fliermans; E L Schmidt
Journal:  Appl Microbiol       Date:  1975-10

5.  Application of the fluorescent-antibody technique to the study of a methanogenic bacterium in lake sediments.

Authors:  R F Strayer; J M Tiedje
Journal:  Appl Environ Microbiol       Date:  1978-01       Impact factor: 4.792

6.  Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material.

Authors:  B B Bohlool; T D Brock
Journal:  Appl Microbiol       Date:  1974-07
  6 in total
  4 in total

1.  Separation and purification of bacteria from soil.

Authors:  L R Bakken
Journal:  Appl Environ Microbiol       Date:  1985-06       Impact factor: 4.792

2.  Autecology in Rhizospheres and Nodulating Behavior of Indigenous Rhizobium trifolii.

Authors:  D H Demezas; P J Bottomley
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

3.  Release of Rhizobium spp. from Tropical Soils and Recovery for Immunofluorescence Enumeration.

Authors:  M T Kingsley; B B Bohlool
Journal:  Appl Environ Microbiol       Date:  1981-08       Impact factor: 4.792

4.  Transposon Tn5 as an identifiable marker in rhizobia: Survival and genetic stability of Tn5 mutant bean rhizobia under temperature stressed conditions in desert soils.

Authors:  S D Pillai; I L Pepper
Journal:  Microb Ecol       Date:  1991-12       Impact factor: 4.552
  4 in total

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