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Literature DB >> 16345464

Alkaline phosphatase assay for freshwater sediments: application to perturbed sediment systems.

Abstract

The p-nitrophenyl phosphate hydrolysis-phosphatase assay was modified for use in freshwater sediment. Laboratory studies indicated that the recovery of purified alkaline phosphatase activity was 100% efficient in sterile freshwater sediments when optimized incubation and sonication conditions were used. Field studies of diverse freshwater sediments demonstrated the potential use of this assay for determining stream perturbation. Significant correlations between phosphatase and total viable cell counts, as well as adenosine triphosphate biomass, suggested that alkaline phosphatase activity has utility as an indicator of microbial population density and biomass in freshwater sediments.

Entities: Chemical

Year: 1979 PMID： 16345464 PMCID： PMC243610 DOI： 10.1128/aem.38.5.922-927.1979

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

4 in total

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Authors: A TORRIANI
Journal: Biochim Biophys Acta Date: 1960-03-11

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Authors: T Berman
Journal: Nature Date: 1969-12-20 Impact factor: 49.962

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Authors: G S Sayler; L C Lund; M P Shiaris; T W Sherrill; R E Perkins
Journal: Appl Environ Microbiol Date: 1979-05 Impact factor: 4.792

4. Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa.

Authors: K J Cheng; J M Ingram; J W Costerton
Journal: J Bacteriol Date: 1971-07 Impact factor: 3.490

4 in total

12 in total

1. Effects of alkaline phosphatase activity on nucleotide measurements in aquatic microbial communities.

Authors: D M Karl; D B Craven
Journal: Appl Environ Microbiol Date: 1980-09 Impact factor: 4.792

2. Seasonal and spatial distribution of extracellular enzymatic activities and microbial incorporation of dissolved organic substrates in marine sediments.

Authors: L A Meyer-Reil
Journal: Appl Environ Microbiol Date: 1987-08 Impact factor: 4.792

3. Relationship between microbial activity of stream sediments, determined by three different methods, and abiotic variables.

Authors: J H Baker
Journal: Microb Ecol Date: 1986-06 Impact factor: 4.552

4. Impact of coal-coking effluent on sediment microbial communities: a multivariate approach.

Authors: G S Sayler; T W Sherrill; R E Perkins; L M Mallory; M P Shiaris; D Pedersen
Journal: Appl Environ Microbiol Date: 1982-11 Impact factor: 4.792

5. Microcosm and experimental pond evaluation of microbial community response to synthetic oil contamination in freshwater sediments.

Authors: G S Sayler; R E Perkins; T W Sherrill; B K Perkins; M C Reid; M S Shields; H L Kong; J W Davis
Journal: Appl Environ Microbiol Date: 1983-07 Impact factor: 4.792

6. Rates of microbial transformation of polycyclic aromatic hydrocarbons in water and sediments in the vicinity of a coal-coking wastewater discharge.

Authors: S E Herbes
Journal: Appl Environ Microbiol Date: 1981-01 Impact factor: 4.792

7. Perspectives on the use of algae as biological indicators for monitoring and protecting aquatic environments, with special reference to malaysian freshwater ecosystems.

Authors: Wan Maznah Wan Omar
Journal: Trop Life Sci Res Date: 2010-12